| Project/Area Number |
11670425
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Legal medicine
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| Research Institution | Osaka City University |
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Principal Investigator |
MAEDA Hitoshi Medical School, Osaka City University Professor, 大学院・医学研究科, 教授 (20135049)
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| Co-Investigator(Kenkyū-buntansha) |
ZHU Bao-li Medical School, Osaka City University Research Associate, 大学院・医学研究科, 助手 (30305619)
FUJITA Masaki Medical School, Osaka City University Lecturer, 大学院・医学研究科, 講師 (00211524)
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| Project Period (FY) |
1999 – 2001
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | Pulmonary surfactant-associated protein A / Pulmonary failure / Acute respiratory distress syndrome / Cause of death / Asphyxiation / Alveolar injury / Hyaline membrane disease / Survival time / 肺サーファクタント関連アポタンパクA / 肺傷害 / 中毒 / 熱傷 |
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| Research Abstract |
The objective of the present research was to assess pulmonary surfactant-associated protein A (SP-A) as a possible marker of pulmonary failure/respiratory distress to establish the forensic pathological criteria in postmortem investigation of death. A series of immunohistochemical and biochemical investigations of SP-A were performed in routine casework. On the basis of the comprehensive analyses of the accumulated data, a pathological classification of the types and severity of pulmonary failure/respiratory distress was proposed : a) acute asphyxiation/respiratory distress of peripheral origins, characterized by a high immunohistochemical score of intra-alveolar granular SP-A and/or an elevation in the ratio of subclass-gene SP-A1/A2 mRNA in the lung tissues ; b) alveolar injury, accompanied by an increase in immunohistochemical membranous SP-A score and/or an elevation in the serum SP-A level ; c) a combination of the above-mentioned two types ; d) protracted respiratory distress, sh
… More
owing intra-alveolar coarse granular/membranous SP-A and an elevation in the serum SP-A level (acute respiratory distress syndrome/hyaline membrane disease). Further investigations to differentiate the primary and secondary origins of respiratory distress suggested the possible usefulness of immunohistochemical ubiquitin positivity in the pigmented neurons of the substantia nigra and serum uric acid level for diagnosis of asphyxiation and drowning, serum creatinine for a marker of thermal and hypoxic skeletal muscle damage, serum and pericardial cardiac troponin-T levels for a marker of myocardial damage. As described above, practical criteria were established for forensic pathological assessment of pulmonary failure/respiratory distress. Furthermore, a possibility of evidence based assessment' using multiple biochemical markers in postmortem investigation of the cause of death, survival time and the related pathophysiology involving respiratory, circulatory and central nervous system dysfunction was suggested. Less
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