| Project/Area Number |
11670435
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Chiba University School of Medicine |
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Principal Investigator |
KOHNO Yoichi School of Medicine Chiba University, Professor, 医学部, 教授 (60161882)
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| Co-Investigator(Kenkyū-buntansha) |
SHIMOJO Naoki School of Medicine Chiba University, Assistant, 医学部, 助手 (40221303)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Homing receptor / alpha E beta 7 integrin / cutaneous lymphocyte antigen / alpha 4 integrin / asthma / food allergy / atopic dermatitis / flow cytometry / cord blood / T cells |
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| Research Abstract |
We found that levels of expression of integrin alpha 4 on Dermatophagoides farinae (Df) antigen-stimulated peripheral blood T cells correlated with disease severity in children with Df-sensitive asthma. The results indicate the importance of homing receptor on allergen-reactive T cells in the pathophysiology of allergic diseases. In order to investigate the mechanisms of involvement of selective target organs in food allergy which is a very common allergic disease in ealry childfood, we studied expression of alpha E beta 7 integrin and cutaneous lymphocyte antigen (CLA) on T cells stimulated with cow's milk protein and hen egg white protein in food-related atopic dermatitis children. CLA was induced on peripheral T cells only in patients with food-allergic dermatitis patients in whom diagnosis was confirmed by food challenge test indicating the involvement of CLA.In contrast, no alpha E beta 7 integrin was induced in these subjects. The results indicate the distinct role of CLA in the pathophysiology in atopic dermatiitis. We thus studied the expression of CLA and alpha E beta 7 integrin on cord blood T cells upon stimulation with milk protein and subsequent development of milk-induced dermatitis. Very interstingly alpha E beta 7 integrin but not CLA was induced on cord blood T cells in some babies and this induction predict subsequent developnment of milk-induced dermatitis. Based on these findings we propose that alpha E beta 7 ibtegrin out food-reactive T cells is a key homing receptor in pathogensis of food-induced dermatitis in young infants.
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