| Project/Area Number |
11670439
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | The University of Tokyo |
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Principal Investigator |
TAKAHASHI Takashi Institute of Medical Science, The University of Tokyo Assistant Professor, 医科学研究所, 助手 (00292855)
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| Co-Investigator(Kenkyū-buntansha) |
IWAMOTO Aikichi Institute of Medical Science, The University of Tokyo Professor, 医科学研究所, 教授 (10133076)
NAKAMURA Yoshikazu Institute of Medical Science, The University of Tokyo Professor, 医科学研究所, 教授 (40114590)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Pneumocystis carinii / Hospital-acquired infections / Drug-resistance / Molecular biology |
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| Research Abstract |
I.Genotyping of Pneumocystis carinii (Pc) isolated from 24 BALF in Japan was examined based on nucleotide sequence variations in internal transcribed spacer regions 1 and 2 (ITS1 and ITS2, respectively) of rRNA genes. We found 11 ITS1 genotypes including 2 novel ones and 11 ITS2 genotypes including 3 new ones. Combination of ITS1 and ITS2 genotypes resulted in 30 ITS genotypes, of which 10 were newly described in this report. Two or more genotypes in ITS regions in a specimen were observed in 16 of 24 patients. Our results will be of help for the epidemiological investigation of Pc infection. II.We examined mutations in dihydropteroate synthase (DHPS) gene of Pc isolated from 24 patients with Pc pneumonia (PCP) in Japan. DHPS mutations were identified at amino acid positions 55 and/or 57 in isolates from 6 (25.0 %) of 24 patients. Co-trimoxazole treatment failed more frequently in patients whose isolates had DHPS mutations than in those whose isolates had wild-type DHPS (n=4 [100%] versus n=2 [11.1%] ; P=0.002). Our results thus suggest that DHPS mutations may contribute to failures of co-trimoxazole treatment for PCP.
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