• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Effects of a Sjogren's syndrome-associated mutation of the TAP2 gene on the diversity of HLA-bound antigen peptides

Research Project

Project/Area Number 11670446
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field 内科学一般
Research InstitutionKobe University

Principal Investigator

KUMAGAI Shunichi  Kobe University School of Medicine, Professor, 医学部, 教授 (00153346)

Co-Investigator(Kenkyū-buntansha) MORINOBU Akio  Kobe University School of Medicine, Assistant, 医学部, 助手 (10294216)
KONDO Shinichi  Kobe University Hospital, Lecturer, 医学部・附属病院, 講師 (50153721)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsTAP2 Gene / antigen peptides / Sjogren's syndrome / peptide transporter / HLA
Research Abstract

1) Preparation of the mutated TAP2 gene.
The A to G mutation was introduced at the 1729^<th> base of TAP2 gene by the method of Shuldier. A.R.et al (Analyt. Biochem. 194, 9-15, 1991), and the mutated (TAP2^*Bky2) and wild (TAP2^*b) type cDNA were prepared.
2) Esablishment of TAP1 and TAP2 genes transfected cell lines.
TAP1 and TAP2 genes (TAP2^*B or TAP2^*Bky2) were transfected to TAP genes-deficient cell line, T2, by electroporation method. Two cell lines. T2/Bky2 and T2/B, which were different only at the codon 577, were established.
3) Collection of HLA-bound peptides from the established cell lines
Antigen peptides bound to HLA-B5 were collected by anti-HLA column using anti-HLA-B5 monoclonal antibody (4D12). After filtration with 5,000 dalton cut-off filter, peptide fraction was collected.
4) Analysis of HLA-bound peptides
The fraction was applied on a reversed-phase HPLC, and some peaks were collected. The repertory and amino acid sequence of HLA-bound peptides are now further analyzed by mass-spectrometry.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (26 results)

All Other

All Publications (26 results)

  • [Publications] Akio Morinobu: "Association of glutathione S-transferase M1 homozygous null genotype with disease susceptibility to Sjogren's syndrome in Japanese."Arthritis Rheum.. 42・12. 2612-2615 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shinichi Kondo: "Factor XII Tenri, a novel cross-reacting material negative factor XII deficiency. occurs through a proteasome-mediated degradation."Blood.. 93・12. 4300-4305 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuyasu Saigo: "Transfusion-related acute lung injury in an acute myelogeneous leukemia patient having anti-IgA2m (1) antibody."J Int Med Res. 27. 96-100 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akio Morinobu: "Bucillamine suppresses human Th1 cell development by a hydrogen peroxide-independent mechanism."J.Rheumatol.. 27・4. 851-858 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Katsuyasu Saigo: "Estimation of Stem Cell Fractions in Peripheral Blood Stem Cell Harvest by Using an SE-9000 Hematology Analyser."Acta Haematologica.. 103. 157-161 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Zheng-Yu Wang: "Mannose-binding Lectin Gene Polymorphisms in Patients with Sjogren's Syndrome."Ann Rheum Dis. (in press).

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shunichi Kumagai: "Proceedings of the "Congress of Pathology and Laboratory Medicine-XX World IV Mercosul-XXXIII Brazilian""Edited by Evsldo Melo. Bologna (Italy), Monduzzi Editore. 300(101-106) (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shunichi Kumagai: "Autoantigens and autoantibodies : Diagnostic tools and clues to understanding autoimmunity."Edited by K.Conrad,R.-L.Humbel,M.Meurer,Y.Shoenfeld, and E.M.Tan (Pabst Science Publishers, Berlin). 762(256-257) (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Morinobu A, et al: "Association of glutathione S-transferase M1 homozygous null genotype with disease susceptibility to Sjogren's syndrome in Japanese."Arthritis Rheum.. 42. 2612-2615 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumagai, S,. et al: "Thioredoxin/ADF (adult T cell leukemia-derived factor) as an indicator of oxidative stress."Proceedings of the "Congress of Pathology and Laboratory Medicine - XX World IV Mercosul - XXXIII Brazilian" Edited by Evsldo Melo. Bologna (Italy), Monduzzi Editore. 101-106 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kondo, S.et al: "Factor XII Tenri, a novel cross-reacting material negative factor XII deficiency, occurs through a proteasome-mediated degradation."Blood. 93(12). 4300-4305 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Morinobu, A., et at: "Bucillamine suppresses human Th1 cell development by a hydrogen peroxide-independent mechanism."J.Rheumatol. 27. 851-858 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kumagai, S, et al: "A new allele of transporter associated with antigen procesing gene (TAP2^*Bky2) and glutathione S-transferase (GST) M1 homozygous null genotype were associated with anti-Ro/SS-A autoantibody production."In Autoantigens and autoantibodies : Diagnostic tools and clues to understanding autoimmunity. Edited by K.Conrad, R.-L.Humbel, M.Meurer, Y.Shoenfeld, and E.M.Tan (Pabst Science Publishers, Berlin).. 256-257 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Wang, Z-G et al: "Mannose-binding Lectin Gene Polymorphisms in Patients with Sjogren's Syndrome."Ann Rheum Dis. (in press.).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akio Morinobu: "Bucillamine suppresses human Th1 cell development by a hydrogen peroxide-independent mechanism."J.Rheumatol.. 27・4. 851-858 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Katsuyasu Saigo: "Estimation of Stem Cell Fractions in Peripheral Blood Stem Cell Harvest by Using an SE-9000 Hematology Analyser."Acta Haematologica.. 103. 157-161 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Zheng-Yu Wang: "Mannose-binding Lectin Gene Polymorphisms in Patients with Sjogren's Syndrome."Ann Rheum Dis. (in press).

    • Related Report
      2000 Annual Research Report
  • [Publications] 西郷勝康: "自家末梢血幹細胞採取、および移植時の血中M-CSFの変動"臨床病理. 48・1. 49-53 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shunichi Kumagai: "Autoantigens and autoantibodies : Diagnostic tools and clues to understanding autoimmunity."Edited by K. Conrad,R.-L.Humbel,M.Meurer,Y.Shoenfeld, and E.M.Tan (Pabst Science Publishers, Berlin). 762(256-257) (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Nobuhide Hayashi: "Autoantigens and autoantibodies : Diagnostic tools and clues to understanding autoimmunity."Edited by K.Conrad,R.-L.Humbel,M.Meurer,Y.Shoenfleld, and E. M.Tan (Pabst Science Publishers, Berlin). 762(696-697) (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akio Morinobu: "Association of glutathione S-transferase M1 homozygous null genotype with disease susceptibility to Sjogren's syndrome in Japanese"Arthritis Rheumat.. 42・12. 2612-2615 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shinichi Kondo: "Factor XII Tenri,a novel cross-reacting material negative factor XII deficiency,occurs through a proteasome-mediated degradation"Blood.. 93・12. 4300-4305 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Akio Morinobu: "Bucillamine suppresses human Th1 cell development by a hydrogen peroxide-independent mechanism"J.Rheumatol.. In press.

    • Related Report
      1999 Annual Research Report
  • [Publications] Shunichi Kumagai: "Thioredoxin/ADF(adult T cell leukemia-derived factor)as an indicator of oxidative stress"Proceedings of the "Congress of Pathology and Laboratory Medicine-XX World IV Mercosul-XXXIII Brazilian". in press.

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuyasu Saigo: "Transfusion-related acute lung injury in an acute myelogeneous leukemia patient having anti-IgA2m(1) antibody"J Int Med Res. 27. 96-100 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Katsuyasu Saigo: "Successful treatment with all-trans retinoic acid combined with small doses of cytosine arabinoside for an aged patient with acute promyelocytic leukemia"Medical Postgraduates.. 37・5. 380-382 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi