Regulatory mechanisms on human eosinophil differentiation
| Project/Area Number |
11670461
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | Toho University |
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Principal Investigator |
TOMIOKA Hisao School of Medicine, Department of Medicine Professor, 医学部, 教授 (20009632)
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| Co-Investigator(Kenkyū-buntansha) |
OCHIAI Kenichi School of Medicine, Department of Medicine Assistant, 医学部, 助手
KAGAMI Masaru School of Medicine, Department of Medicine Assistant, 医学部, 助手 (50233656)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,900,000 (Direct Cost: ¥1,900,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | CD34 cells / eosinophils / IFN-γ / CD69 / JAK2 / STAT1 / p21(Cip-1, WAF-1) / cell cycle / SCF / GM-CSF |
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| Research Abstract |
The purpose of the study is to show how cytokines and interferon (IFN)-gamma regulate eosinophil differentiation. The results of the study are summarized as follows ;
1. 7-28 day of culture with Interleukin-3 (IL-3)-and IL-5 effectively induced eosinophil precursors/eosinophils from human cord blood CD34+ cells.
2. Granulocyte-macrophage colony stimulating factor (GM-CSF) and stem cell factor (SCF) from human umbilical vein endothelial cells promoted IL-3-and IL-5-induced eosinophil differentiation from CD34+ cells.
3. IFN-gamma induced eosinophil survival and CD69 expression through the activation of JAK2 in peripheral eosinophils.
4. IFN-gamma also induced CD69 expression by eosinophil precursors via the activation of JAK2.
5. IFN-gamma induced CD69 expression in the IL-3-and IL-5-induced eosinophils through STAT1.
6. IFN-gamma might inhibit eosinophil apoptosis and arrest the cell cycle by p21 (Cip-1, WAF-1) induction.
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Report
(3 results)
Research Products
(12 results)
