A method to determine antigen peptides for the conally expanded T cells in systemic autoimmune diseases
| Project/Area Number |
11670465
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
内科学一般
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| Research Institution | St.Marianna University School of Medicine |
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Principal Investigator |
KATO Tomohiro Institute of Medical Science, St.Marianna University School of Medicine assistant professor, 難病治療研究センター, 助教授 (80233807)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | systemic autoimmune disease / systemic lupus erythematosus / rheumatoid arthritis / MHC / T cell / antigen peptide / phage / peptide library |
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| Research Abstract |
We previously reported oligoclonal T cell accumulation in the systemic autoimmune diseases. For instance, the T cell clonal expansion was detected in peripheral blood and also in affected kidney in systemic lupus erythematosus (SLE) and in synovial tissue in rheumatoid arthritis (RA). However, target molecules of these accumulated or expanded T cells remains to be determined, even though they are of great importance for understanding the pathogenesis of the diseases. To determine the antigen-peptides of the T cell clonotypes, we tried to establish screening system of antigenic peptides using random peptide phage library with MHC molecules and reconstructed TCR from clinical samples. As a result, we demonstrated that HLAB5101 and H-2Db with betamicroglobulin is expressed on phage with ability to bind antigenic peptides. Further, we obtained TCR a and b chain in pair from one T cell of clinical samples from patients with SLE, using single cell PCR and reconstructed the TCR as a single chain in vitro. Combination of the reconstructed TCR and randomized peptide library would be a powerful tool to determine the antigens for the clonally expanded T cells.
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Report
(3 results)
Research Products
(17 results)
