| Project/Area Number |
11670483
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | NIIGATA UNIVERSITY |
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Principal Investigator |
TAKAHASHI Toru University Medical Hospital, NIIGATA UNIVERSITY, Assistant, 医学部・附属病院, 助手 (20188030)
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| Co-Investigator(Kenkyū-buntansha) |
ICHIDA Takafumi University Medical Hospital, NIIGATA UNIVERSITY, Lecturer, 医学部・附属病院, 講師 (00126509)
MATSUDA Yasunobu University Medical Hospital, NIIGATA UNIVERSITY, Medical Staff, 医学部・附属病院, 医員
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | MutS / plasma DNA / MutS蛋白 |
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| Research Abstract |
It is now being described that detection of mutated DNA using MutS protein is highly sensitive and accurate. because MutS can specifically bind mis-matched double strand of DNA, identifying mutated DNA(e.g., point mutation, deletion and insertion)is capable by referring to the amount of DNA-bound MutS.We addressed whether MutS could be useful in the detection of mutated DNA in the plasma, and examined its usefulness in case of patients affected with pancreatic/bile duct cancers.
We obtained plasma DNA from 25 of patients with pancreatic cancers and 33 with bile duct cancers. PCR was performed using primers specific for p53(exon 5-8)and p16(exon 1-2), and mis-matched heteroduplex was examined using MutS-binding reaction. Consequently, we could detect mutated DNA in 18 and 33 patients with pancreatic cancers and bile duct cancers, respectively. Thus, we surmise that detecting mutated DNA in the plasma using MutS is useful for identifying the ptients who are suffered from solid cancers.
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