| Project/Area Number |
11670493
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Mie University |
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Principal Investigator |
TAMAKI Shigenori Mie University, Hospital, Assistant, 医学部・附属病院, 助手 (80260602)
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| Co-Investigator(Kenkyū-buntansha) |
ESTEBAN CESAR Gabazza Mie University, Faculty of Medicine, Assistant, 医学部, 助手 (00293770)
KARIBAYASHI Kagemasa Mie University, Faculty of Medicine, Professor, 医学部, 教授 (10064578)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | HCV / CTL / vaccine / mouse model / HCV / CTL |
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| Research Abstract |
1.Establishment of novel animal model of hepatitis C virus(HCV)infection
BALB/c mice were intrahepatically inoculated with plasmid DNA encoding HCV structural proteins(core, E1 and E2). Expression of HCV structural proteins in hepatocytes were detected immunohistochemically 16 weeks after inoculation. These intrahepatically DNA-inoculated mice developed CD8^+, MHC class I-restricted cytotoxic T lymphocytes(CTL)resposes against all three HCV structural proteins.
2.Induction of HCV-specific CTL by HCV vaccines
(1)DNA waccine
We assessed the capability of a novel plasmid, pEFCE1E2, encoding HCV structural proteins(core, E1 and E2)under the elongation factor 1-α promoter to generate CTL aganinst HCV.BALB/c mice were immunized with the pEFCE1E2 but not with a plasmid possessing the same cDNA under the cytomegalovirus promoter developed HCV-specific effector cells by a single immunization.
(2)peptide waccine
We explored the use of epitope peptides by in vivo electroporation to introduce directly into the cytoplasm for the vaccine elicitation of virus specific CTL.BALB/c mice were immunized with HCV NS5(P17, residues 2423-2434)with or without electric pulses. HCV-specific effector cells were elicited in mice immunized with peptide with electric administration but not without electric administration.
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