• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Analysis of susceptible genes of fatty liver

Research Project

Project/Area Number 11670503
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionOsaka University

Principal Investigator

YAMATO Eiji  Osaka University Graduate School of Medicine, Associate Professor, 医学系研究科, 助教授 (20273667)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsfatty liver / susceptible genes / subtraction method / the NSY mouse / サブトラクションクローニング / ポジショナルクローニング
Research Abstract

We have developed NSY mice which are models of type 2 diabetes mellitus. These mice are also developed fatty liver in time-dependent manner. In this research, we have isolated the genes which showed the differential expression in the fatty liver of NSY mice in comparison with the genes expressed in the liver in C3H/He mice using PCR-based cDNA subtraction method which we had already developed. The earlier method analyzed the BamHI fragment to avoid the undesirable bias of PCR-amplification by difference of DNA length. However this method may cause pseudo-negative cDNA because only BamHI fragments had been analyzed. Thus, in this study, we analyzed not only BamHI fragments, but also HindIII fragments of cDNA of the liver.
Results
BamHI fragment
NSY liver dominant-genes : 3 clones, glucuronosyltransferase, human EST, 1 unknown gene
C3H dominant-genes : 4 clones, TFII-I, DRPLA, collagen typeIV, 1 unknown gene
HindIII fragment
NSY liver dominant-genes : 3 unknown clones
C3H dominant-genes : 5 clones, Ser/Thr phosphatase, HMG CoA reductase, translation initiation factor, IAP, 1 unknown gene
Along with this subtraction analysis, the study on the position of the susceptible gene of fatty liver by linkage analysis using the crossing of NSY mice with C3H/He mice are now on going. The results of the subtraction analysis will lead a susceptible gene for fatty liver in combination with the linkage analysis.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Ueda H: "Genetic analysis of late-onset type 2 diabetes in a mouse model of human complex trait."Diabetes. 48. 1168-1174 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujisawa T: "Association of plasma fibrinogen level and blood pressure with diabetic retinopathy, and renal complication associated with proliferative diabetic retinopathy in type 2 diabetes mellitus."Diabetic Med. 16. 522-526 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hattori M: "Homologous recombination of the MHC class I K region defines new MHC-linked diabetogenic susceptibility gene (s) in NOD mice."J Immunol. 163. 1721-1724 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujisawa T: "Length rather than a specific allele of dinucleotide repeat in 5' upstream region of aldose reductase gene is assoiciated with diabetic retinopathy."Diabetic Med. 16. 1044-1047 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda H: "Age-dependent changes in phenotypes and candidate gene analysis in a polygenic animal medel of type II diabetes ; NSY mouse."Diabetologia. 43. 932-938 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda H: "Paternal-maternal effects on phenotypic characteristics in spontaneously diabetic Nagoya-Shibata-Yasuda mice."Matabolism. 49. 651-656 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda H.: "Genetic analysis of late-onset type 2 diabetes in a mouse model of human complex trait."Diabetes. 48. 1168-1174 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujisawa T.: "Association of plasma fibrinogen level and blood pressure with diabetic retinopathy, and renal complication associated with proliferative diabetic retinopathy in type 2 diabetes mellitus."Diabetic Med. 16. 522-526 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hattori M.: "Homologous recombination of the MHC class I K region defines new MHC-linked diabetogenic susceptibility gene (s) in NOD mice."J Immunol. 163. 1721-1724 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fujisawa T.: "Length rather than a specific allele of dinucleotide repeat in 5' upstream region of aldose reductase gene is associated with diabetic retinopathy."Diabetic Med. 16. 1044-1047 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda H.: "Age-dependent changes in phenotypes and candidate gene analysis in a polygenic animal medel of type II diabetes ; NSY mouse."Diabetologia. 43. 932-938 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda H.: "Paternal-maternal effects on phenotypic characteristics in spontaneously diabetic Nagoya-Shibata-Yasuda mice."Matabolism. 49. 651-656 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hotta M.: "Oxidative stress and pancreatic β-cell destruction in insulin-dependent diabetes mellitus."In "Antioxidants in Diabetes Manegement", Packer L, Rosen P, Tritschler HJ, King *L, Azzi A eds., Marcel Dekker, New York. 265-274 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kawamoto S.: "Pancreatic islet-specific expression of viral interleukin-10 prevents autoimmune diabetes in nonobese diabetic mice : Differential effect of viral IL-10 vs. murine IL-10."J Immunol. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ueda H: "Genetic analysis of late-onset type 2 diabetes in a mouse model of human complex trait."Diabetes. 48. 1168-1174 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Hattori M: "Homologous recombination of the MHC class I K region defines new MHC-linked diabetogenic susceptibility gene(s) in NOD mice."J Immunol. 163. 1721-1724 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujisawa T: "Association of plasma fibrinogen level and blood pressure with diabetic retinopathy, and renal complication associated with proliferative diabetic retinopathy in type 2 diabetes mellitus."Diabetic Med. 16. 522-526 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Fujisawa T: "Length rather than a specific allele of dinucleotide repeat in 5'upstream region of aldose reductase gene is associated with diabetic retinopathy."Diabetic Med. 16. 1044-1047 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ueda H: "Age-dependent changes in phenotypes and candidate gene analysis in a polygenic animal medel of type II diabetes ; NSY mouse."Diabetologia. 43. 932-938 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ueda H: "Paternal-maternal effects on phenotypic characteristics in spontaneously diabetic Nagoya-Shibata-Yasuda mice."Matabolism. 49. 651-656 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ueda H.et.al.: "Genetic analysis of late-onset type 2 diabetes in a mouse model of human complex trait"Diabetes. 48. 1168-1174 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi