Association of hepatic oval cell and liver diseses : implication to new therapy

• Project/Area Number: 11670504
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Gastroenterology
• Research Institution: Tottori University
• Principal Investigator: KAWASAKI Hironaka Tottori University, Faculty of Medicine, Second Department of Internal Medicine, Professor, 医学部, 教授 (60108826)
• Co-Investigator(Kenkyū-buntansha): SHIOTA Goshi Tottori University, Faculty of Medicine, Second Department of Internal Medicine, Assistant Professor, 医学部・附属病院, 講師 (70263457)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥3,900,000 (Direct Cost: ¥3,900,000); Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000); Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
• Keywords: hepatic oval cell / fulminant hepatitis / HGF / gene therapy / signal transduction

Research Abstract

To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt- Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased increased at each time point. The results suggest that in vivo transfer of hepatic oval cells in the Solt-Farber model in rats.
Recently, we examined the effect of pAxCAHGF on OC/CDE6 cells, hepatic oval cells. The OC/CDE6 cells, which are infected by pAxCAHGF, grow more rapidly than those by pAXCALacZ.The pathway via which signal of HGF is transmitted is mainly PI3-K, especially Akt. The MAPK is associated with the signal transduction of HGF, but not a main pathway.

Research Products

• [Publications] Goshi Shiota et al.: "Invivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oral cells in a 2-acetylaminoflurene/patial hepatectomy modelin rats"FEBS Letters. 470. 325-330 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Goshi Shiota, Takahiro Kunisada, Kenji Oyama, Akihide Udagawa, Takahiro Nomi, Kiwamu Tanaka, Atsushi Tsutsumi Nakamura, Hirofumi Mamada, Takashi Sakatani: "Stewaqrt Sell, Kenzo Sato, Hisao Ito, Hironaka Kawasaki. In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cell in a 2-acetylaminofluorene/partial hepatectomy model in rats."FEBS Letters. 470. 325-330 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Goshi Shiota et al.: "In vivo transfer of hepatocyte growth factor gene accelerates proliferation of hepatic oval cells in a 2-acetylaminofluorene/partial hepatectomy model in rats."FEBS Letters. 470. 325-330 (2000)
• [Publications] Goshi Shiota,etal.: "In vivo tsansfer of hepatocyte growth factor gene accelerates prsliferation of hepatic oval cells in a 2-acety lamino fluorme/partial"FEBS Letters. (in press). (2000)