| Project/Area Number |
11670504
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Tottori University |
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Principal Investigator |
KAWASAKI Hironaka Tottori University, Faculty of Medicine, Second Department of Internal Medicine, Professor, 医学部, 教授 (60108826)
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| Co-Investigator(Kenkyū-buntansha) |
SHIOTA Goshi Tottori University, Faculty of Medicine, Second Department of Internal Medicine, Assistant Professor, 医学部・附属病院, 講師 (70263457)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,900,000 (Direct Cost: ¥3,900,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | hepatic oval cell / fulminant hepatitis / HGF / gene therapy / signal transduction |
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| Research Abstract |
To clarify the effect of hepatocyte growth factor (HGF) on proliferation of hepatic oval cells, we transferred HGF gene into liver of the Solt- Farber rat model. Male Fisher 344 rats were infected with a recombinant adenovirus carrying the cDNA for HGF (pAxCAHGF) from tail vein. HGF mRNA showed its peak at 4 days, and diminished thereafter. The total and proliferating cell nuclear antigen-positive hepatic oval cells were significantly elevated in HGF-transferred rats, in which stem cell factor and c-kit mRNA increased increased at each time point. The results suggest that in vivo transfer of hepatic oval cells in the Solt-Farber model in rats.
Recently, we examined the effect of pAxCAHGF on OC/CDE6 cells, hepatic oval cells. The OC/CDE6 cells, which are infected by pAxCAHGF, grow more rapidly than those by pAXCALacZ.The pathway via which signal of HGF is transmitted is mainly PI3-K, especially Akt. The MAPK is associated with the signal transduction of HGF, but not a main pathway.
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