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Prevention of lymphotropic hepatitis C virus infection by blocking antibodies

Research Project

Project/Area Number 11670512
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKYUSHU UNIVERSITY

Principal Investigator

HAYASHIDA Kazuhiro  Kyushu University Hospital, Assistant, 医学部・附属病院, 助手 (60180981)

Co-Investigator(Kenkyū-buntansha) ISHIBASHI Hiromi  Kyushu University Graduate School of Medical Science, Ass.Prof., 大学院・医学研究院, 助教授 (80127969)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
KeywordsChronic hepatitis C / HCV / Cell tropism / lymphocyte / blocking antibody / interferon
Research Abstract

We measured the binding ability of chronic hepatitis C virus (HCV) to the lymphocyte cell line HPB-Ma in the patient's serum, and found that it was one of the major determinant of interferon therapy by the prospective study of 152 consecutively enrolled patients (J Hepatology). As the HCV loose their infectivity by neutralized antibodies, we measured the quantity of antibody-free virion in the serum, and determined the amino acid sequence of hypervariable region of envelope 2 protein for the 38 patients ; 22 genotype 1b and 16 genotype 2a/2b. We found the consensus sequence of hypervariable region in the genotype 1b patients with a small amount of antibody-free virion and the all patients with a genotype 2a/2b infection regardless of the quantity of antibody-free virion, but not in the genotype 1b patients with a high amount of antibody-free virions (J Med Virol).
To determine the cell tropism of HCV, we established the measurement method of HCV that bound to the hepatocyte cell line HuH7. The HCV in the majority of patients could bind to the both cell lines HPB-Ma and HuH7, while the HCV in some patients could bind only to the hepatocyte cell line HuH7. There are no HCV that could bind only to the lymphocyte cell line HPB-Ma. The interferon treatment efficacy was well related not to the binding ability to the hepatocyte cell line HuH7 but to the lymphocyte cell line HPB-Ma. Now, we are examining the hypervariable region to analyze the cell tropism and the blocking antibody against the lymphocyte infection.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (7 results)

All Other

All Publications (7 results)

  • [Publications] Yoichi Kimura et al.: "Antibody-free virion titer greatly differs betwean hepatitis C virus genotypes"Journal of Medical Virology. 61・1. 37-43 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoichi Kumura et al.: "Low cell binding ability of HCV is closely related to interferon treatment especially in patients with HCV gonotype Za /Zb : a large series prospective study on Japanese patients with chrenic hepatitis C"Journal of Hepatology. 33・5. 818-825 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kimura Y, Hayashida K, Ishibashi H, Niho Y, Yanagi Y: "Antibody-free virion titer greatly differs between hepatitis C virus genotypes"J Med Virol. 61(1). 37-43 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kimura Y, Hayashida K, Yanagi Y, Ishibashi H, Akazawa K, Niho Y: "Low cell binding ability of HCV is closely related to interferon treatment especially in patients with HCV genotype 2a/2b : a large series prospective study on Japanese patients with chronic hepatitis C"J Hepatology. 33(5). 818-825 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoichi Kimura et al.: "Antibody-free virion titer greatly differs between hepatitis C virus genotypes"Journal of Medical Virlolgy. 61・1. 37-43 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoichi Kimura et al.: "Low cell bonding ability of HCV is closely related to interferron treatment especially in patients with HCV genotype 2a/2b : a large series prospective study on Japanese patients with chronic hepatitis C"Journal of Hepatology. 33・5. 818-825 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoichi Kimura: "Antibody-Free Virion Titer Greatly Differs Between Hepatitis C Virus Genotypes"Journal of Medical Virology. 61. (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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