| Project/Area Number |
11670528
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Gastroenterology
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| Research Institution | Jichi Medical School |
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Principal Investigator |
MORIYAMA Takashi Jichi Medical School, Department of Medicine, Assistant Professor, 医学部, 講師 (10240706)
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| Co-Investigator(Kenkyū-buntansha) |
KANEKO Takashi Jichi Medical School, Department of Medicine, Assistant, 医学部, 助手 (10254913)
|
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| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥600,000 (Direct Cost: ¥600,000)
Fiscal Year 1999: ¥3,000,000 (Direct Cost: ¥3,000,000)
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| Keywords | hepatitis C virus / DNA vaccine / cytotoxic T lymphocyte / dendritic cell |
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| Research Abstract |
We tried to induce a T cell response to hepatitic C virus (HCV) NS5A protein by using a DNA vaccine in mice. We immunized mice with a plasmid encoding HCV NS5A region alone or with an adjuvant plasmid encoding IL-2, GM-CSF, or CD86. Neither of them induced a cytotoxic T lymphocyte (CTL) response of a siginificant magnitude.
Next we induced mature dendritic cells with IL-4 and GM-CSF from bone marrow cells. We immunized mice with dencritic cells pulsed with reportedly immunogenic CTL epitopes. None of these mice showed a better T cell response yet.
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