| Project/Area Number |
11670539
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Gastroenterology
|
|---|
| Research Institution | Jikei University School of Medicine |
|---|
Principal Investigator |
FURUSAKA Akihiro Jikei Univercity School of Medicine Assistant Proffesor, 医学部, 講師 (00266678)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SHIRAKI Katsuya Mie Univercity School of Medicine Instructor, 医学部, 助手 (90263003)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,400,000 (Direct Cost: ¥3,400,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
|
|---|
| Keywords | Tumor Suppressor Gene / HBx |
|---|
| Research Abstract |
Using the yeast two hybrid system of HBx, a 2619 bp gene was isolated and had putative Initiation methionine residues at 15 bp and stop codon at 1375 bp (encoding 453 amino acids). We have named this protein, X associating factor-1 (XAF-1). We made the GST-HBx, XAF-1 andmade sure the each other binding with in vitro binding assay. A human nothern blotting identified mRNA expression present in various tissues ubiquitously especially smooth muscle, liver and heart. Similarity searches showed that XAF-1 exibits 57% gene sequence identity to Drosophila melanogaster tumor suppressor gene (Tid gene). On the same period, other group reported a new tumor suppressor gene named hTid1 binding with papiloma virus type 16 E7. To comparing XAF-1, it revealed hTid1 was a alternative splicing form of XAF-1, and both genes expressed in many hepatocellular carcinoma cell lines ubiquitously. To characterize XAF-1, we overexpressed XAF-1 in HepG2 cells and stimulated Anti-Fas Ab, adriamycine and revealed that XAF-1 accelerated the cell apoptosis in both experiments. We hyposised that HBx bindinding to XAF-1 change the function of XAF-1 and causing hepatocarcinogenesis.
|
