• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

The role of new ABC transporter in anti-cancer drug resistance in hepatoma.

Research Project

Project/Area Number 11670547
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Gastroenterology
Research InstitutionKansai Medical University

Principal Investigator

NAKAHASHI Yoshitsugu  Kansai Medical University Medicine Instructor, 医学部, 助手 (70247930)

Co-Investigator(Kenkyū-buntansha) MIYAZAKI Hiroaki  Kansai Medical University Medicine Instructor, 医学部, 助手 (30268370)
NAITOH Yuji  Kansai Medical University Medicine Assistant Professor, 医学部, 講師 (30198014)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
KeywordsResistance to anti-cancer drug / ABC transporter / Hepatoma
Research Abstract

1.Expression of anthracycline resistance-associated protein (ARA) and multidrug resistance-associated protein 6 (MRP6) RNA in hepatoma cell lines.
ARA and MRP6 were detected by RT-PCR and expressed by Northern blot analysis in HepG2, Huh7, PLC/PRF/5, HCC-T and HCC-M cell lines.
2.Increased expression of ARA and MRP6 by anti-cancer drugs.
HepG2, Huh7, PLC/PRF/5 were treated with 10ng/ml epirubicin for 7 days. ARA and MRP6 were not induced in these cell lines for 7 days.
3.Amplification of ARA and MRP6 gene in anti-cancer resistant hepatoma cell lines.
We established 100ng/ml epirubicin-resistant Huh7 cell line. Southern blot analysis indicated that both of ARA and MRP6 gene were amplified in this Huh7 cell line.
4.Modulation of anti-cancer drug resistance by xenobiotics excreted through MRP
Glycyrrhizin is known to be the MRP2 substrate. Glycyrrhizin enhanced anti-cancer effect of epirubicin when HepG2, Huh7 and PLC/PRF/5 were treated with epirubicin and glycyrrhizin.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (6 results)

All Other

All Publications (6 results)

  • [Publications] Taketani S.: "Regulation of the expression of human ferrochelatase by intracellular iron levels."Eur.J.Biochem.. 267. 4685-4692 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yanagitani S.: "Ischemia induces metallothionein III expression in neurons of rat brain."Lift Sciences. 64. 707-715 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yanagitani S., Miyazaki H., Nakahashi Y., Kuno K., Ueno Y., Matsushita M., Naitoh Y., Taketani S.and Inoue K.: "Ischemia induces metallothionein III expression in neurons of rat brain."Life Sciences. 64. 707-715 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Taketani S., Adachi Y., and Nakahashi Y.: "Regulation of the expression of human ferrochelatase by intracellular iron levels."Eur.J.Biochem.. 267. 4685-4692 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Taketani S.: "Regulation of the expression of human ferrochelatase by intracellular iron levels."Eur.J.Biochem.. 267. 4685-4692 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yanagitani S.: "Ischemia induces metallothionein III expression in neurons of rat brain."Life Sciences. 64(8). 707-715 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi