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Positive interaction between tumor cells and tumor stromal cells for tumor growth.

Research Project

Project/Area Number 11670567
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Respiratory organ internal medicine
Research InstitutionChiba University

Principal Investigator

TAKIGUCHI Yuichi  Chiba University, University Hospital, Research Associate, 医学部・附属病院, 助手 (30272321)

Co-Investigator(Kenkyū-buntansha) KIMURA Hiroshi  Chiba University School of Medicine, Associate Professor, 医学部, 助教授 (20195374)
KURIYAMA Takayuki  Chiba University School of Medicine, Professor, 医学部, 教授 (20009723)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,700,000 (Direct Cost: ¥1,700,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
Keywordslung carcinoma / tumor stroma / fibroblast / hepatocyte growth factor / vascular endothelial growth factor / gene therapy / vascular endothelial growth factor / 肺小細胞癌 / HGF / VEGF
Research Abstract

To establish an experimental model for investigating interaction between tumor and its stromal fibroblasts, the research utilized a human small cell lung carcinoma cell line, Wa, and a mouse fibroblast cell line, W-fib.
As results, 1) The model, in which a positive reciprocal interaction between tumor cells and fibroblasts is investigated both in vitro and in vivo, was established. 2) In vitro co-culture of W-fib with Wa obviously enhanced plating efficiency of the Wa. Some soluble factors released from the fibroblasts were speculated to cause the effect.3) The W-fib also enhanced tumorigenesity of the Wa when inoculated into nude mice, by reducing incubation period.4) In vitro co-culture of Wa and W-fib produced high level hepatocyte growth factor (HGF) with a peak at 18 hours after the culture, followed by high level vascular endothelial growth factor (VEGF) with a peak at 27 hours in the culture supematant. The Wa alone or W-fib alone, however, did not prodece HGF or VEGF in the culture medium. 5) An antisense oligo DNA targeting HGF blocked the production of VEGF, and, thereafter, suppresed plating efficinecy of the Wa, in vitro.
In conclusion, in the model presented here, a positive reciprocal interaction between the tumor cells and their stromal fibroblasts was demonstrated. This phenomenon was associated with HGF production by the stromal fibroblasts and VEGF production by the tumor cells.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (21 results)

All Other

All Publications (21 results)

  • [Publications] Takiguch,Yuichi: "Stable pulmonary capillary haemangiomatosis without symptomatic pulmonary bypertension"Thorax. (in press). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Moriya,Tetsuro: "Controlling malignant pericardial effusion by intrapericardial carboplatin administration in patients with primary non-small-cell lung cancer."British Journol of Cancer. 83・7. 858-862 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 渡辺励子: "肺癌の血清腫瘍マーカー"日本臨床. 58・5. 1070-1073 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyazawa,Teruomi: "Implantation of ultraflex nitinol stents in malignant tracheobronchial stenoses."Chest. 118・4. 959-965 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 滝口裕一: "肺野末梢病変に対する、車載型ヘリカルCTの画像評価"肺癌. 39・6. 835-842 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 須田明: "原発性肺癌骨転移診断における血中I型コラーゲン-C-テロペプチド(ICTP)測定の有用性"呼吸. 18・3. 313-317 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takiguchi Y, et al.: "Stable pulmonary capillary haemangiomatosis without symptomatic pulmonary hypertension."Thorax. (in press). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Moriya T, Takiguchi Y, et al.: "Controlling malignant pericardial effusion by intrapericardial carboplatin administration in patients with primary non-small-cell lung cancer"British Journal of Cancer. 83. 858-62 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe, R., Takiguchi, Y., and Kuriyama, T.: "Serum tumor markers for primary lung carcinoma"Japanese Journal of Clinical Medicine. 58. 1070-3 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Miyazawa T, Yamakido M, Ikeda S, Furukawa K, Takiguchi Y, et al.: "Implantation of ultraflex nitinol stents in malignant tracheobronchial stenoses."Chest. 118. 959-965 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takiguchi Y, et al.: "Relevance of mobile CT for detecting and evaluating peripherally located small pulmonary lesions"Japanese Journal for Lung Cancer. 39. 835-842 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Suda A, Takiguchi Y, et al.: "Inflammatory bronchial polyps with a foreign body-like substance"Japanese Journal of Thoracic Diseases.. 35. 965-8 (1997)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takiguchi,Yuichi: "Stable pulmonary capillary haemangiomatosis without symptomatic pulmonary hypertension."Thorax. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mariya, Tetsuro: "Controlling malignant pericardial effusion by intrapericardial carboplatin administration in patients with primary non-small-cell lung cancer."British Journal of Cancer. 83・7. 858-862 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 渡辺励子: "肺癌の血清腫瘍マーカー"日本臨床. 58・5. 1070-1073 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] MiyazAwa, Teruomi: "Implantation of ultraflex nitinol stents in malignant tracheobronchial stenoses."Chest. 118・4. 959-965 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 滝口裕一: "肺野末梢病変に対する、車載型ヘリカルCTの画像評価"肺癌. 39・6. 835-842 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 須田明: "原発性肺癌骨転移診断における血中I型コラーゲン-C-テロペプチド(ICTP)測定の有用性"呼吸. 18・3. 313-317 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Miyazawa T, Takiguchi Y, et al.: "Implantation of Ultraflex mitinol stents in malignant tracheobronchial stenoses"Chest. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] 滝口裕一、et al.: "肺野末梢病変に対する、車載型ヘリカルCTの画像評価"肺癌. 39・6. 835-842 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 須田明、滝口裕一、 et al.: "原発性肺癌骨転移診断における血中I型コラーゲン-C-テロペプチド(ICTP)測定の有用性"呼吸. 18・3. 313-317 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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