| Project/Area Number |
11670572
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | Hamamatsu University School of Medicine |
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Principal Investigator |
SUDA Takafumi Assistant Professor 2^<nd> Div. of Internal Med., Hamamatsu University School of Med., 医学部・附属病院, 助手 (30291397)
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| Co-Investigator(Kenkyū-buntansha) |
CHIDA Kingo Assistant Professor 2^<nd> Div. of Internal Med., Hamamatsu University School of Med., 医学部, 助教授 (40197611)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2001)
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| Budget Amount *help |
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | dendritic cells / granuloma |
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| Research Abstract |
Dendritic cells (DCs) are the most potent antigen-presenting cells and play a central role in initiating the primary immune response. However, their role in granulomatous inflammation has not been determined. The aim of this study was to elucidate the potential role of DCs in granuloma formation. Using a rat model of bacillus Calmette-Guerin (BCG)-elicited pulmonary granulomas, we investigated the distribution of DCs in the granulomas by immunohistochemistry with a rat-DC-specific monoclonal antibody, OX62. We found numerous large, pleiomorphic OX62^+ cells accumulating at the borders of the pulmonary granulomas, and these cells increased in number as the granulomas matured. The OX62^+ cells isolated from the granulomatous lung showed intense surface expression of MHC class II as well as the costimulatory molecules B7-1, B7-2 and a lack of T cell- and monocyte/macrophage-specific markers. Their ultrastructural morphology was characteristic of DCs. Functionally, they had potent capacity to stimulate allogeneic T cells and PPD-specific syngeneic T cells in the absence of exogenous peptides, and expressed high level of IL-12 p40 mRNA. Based on these findings, the OX62^+ cells infiltrating the granulomas were considered to be DCs expressing BCG-derived peptides. These results suggest that DCs participate in pulmonary granuloma formation elicited by BCG through their potent antigen-presenting function and cytokine production, providing a novel insight into DC function during T cell-mediated immune responses.
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