| Project/Area Number |
11670594
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Respiratory organ internal medicine
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| Research Institution | JIKEI UNIVERSITY SCHOOL OF MEDICINE |
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Principal Investigator |
KOJIMA Akira JIKEI UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (60256378)
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| Co-Investigator(Kenkyū-buntansha) |
TSUCHIYA Masashi JIKEI UNIVERSITY SCHOOL OF MEDICINE, RESEARCH ASSOCIATE, 医学部, 助手 (20236912)
YOSHIMURA Kunihiko JIKEI UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部, 講師 (60246452)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,600,000 (Direct Cost: ¥2,600,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,600,000 (Direct Cost: ¥1,600,000)
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| Keywords | LUNG CANCER / FHIT GENE / GENE THERAPY |
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| Research Abstract |
TO EVALUATE THE CONCEPT THAT IN VIVO TRANSFER OF THE HUMAN FHIT GENE WILL SUPPRESS THE LUNG CANCER CELLS, WE CONSTRUCTED AN ADENOVIRUS VECTOR (AdCMVFHIT) CARRYING THE HUMAN FHIT GENE DRIVEN BY THE CMV PROMOTOR.
THE EXPRESSION OF THE FHIT cDNA FOLLOWING IN VITRO TRANSFER WITH THE AdCMVFHIT VECTOR WAS EVALUATED AT THE mRNALEVEL.NORTHERN ANALYSIS REVEALED mRNA TRANSCRIPTS OF THE FHIT cDNA IN A549 CELLS.IN ADDITION, HPLC ANALYSIS SHOWED FUNCTIONAL FHIT PROTEIN RESPONDED WITH AR3A.WE EVALUATED THE GROWTH OF A549 CELLS FOLLOWING INFECTION WITH AdCMVFHIT IN VITRO.THE INFECTED CELLS WERE SIGNIFICANTLY SUPPRESSED IN COMPARISON WITH CONTROL (P<0.05).
CONSISTENT WITH THESE IN VITRO OBSERVATIONS, WHEN AdCMVFHIT WAS DIRECTLY INJECTED INTO ESTABLISHED SUBCUTANEOUS A549 TUMORS IN NUDE MICE, THERE WAS 47% REDUCTION WITH IN TUMOR SIZE AT DAY 20 AND A 51% AT DAY 24 (P=0.023 AND P=0.013).
THESE OBSERVATIONS SUGGEST THAT LOCAL GENE TRANSFER OF THE HUMAN FHIT GENE MAY HAVE POTENTIAL AS A STRATEGY FOR CONTROL OF GROWTH OF LUNG CANCER.
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