| Project/Area Number |
11670618
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Neurology
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
TERUNUMA Hiroshi Yamanashi Medical University, Department of Microbiology, Lecture, 医学部, 講師 (50217436)
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| Co-Investigator(Kenkyū-buntansha) |
IWATANI Yasumasa Yamanashi Medical University, Department of Microbiology, Assistant Professor, 医学部, 助手 (90303403)
KASAI Hirotake Yamanashi Medical University, Department of Microbiology, Assistant Professor, 医学部, 助手 (20324189)
石田 尚臣 東京大学, 医科学研究所, 助手 (80293447)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥1,000,000 (Direct Cost: ¥1,000,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
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| Keywords | Human herpesvirus type 8 / kaposi's sarcoma / AIDS / HIV / cehmokine / vMIP / Zamibia |
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| Research Abstract |
We studied the clinical and immunolgical characteristics of 83 Zombian patients with Kaposis sarcoma(KS)and collected biopsy samples from some of these patients after getting their informed consent. We found thet some of the KS patients still maintained high CD4 counts, but those with an oral lesion(s)had significantly lower CD4 counts. We are now determining the vMIPs nucleotide sequences from biopsy samples for comparison with those of laboratory HHV-8 strains.
To analyze the effects of chemokine-like proteins encoded by the human herpesvirus type 8 on HIV infection, we amplified and isolated viral MIP(vMIP)-I, -II, and -III genes of human herpesvirus type 8(HHV-8)from BCBL-l cell line by PCR.We then constracted these genes into an expression vector and transfected these vectors into a 293T cell line. Culture supernatant containing secreted vMIPs showed the activity of Ca2+ influx to THP-1 cell line. vMIPs genes were also introduced into Cos 7 cells and permanent cell lines secreting vMIPs have been established. We purified a large amount of vMIPs and are studying the effects of these prroteins on HIV infection and immunological effects. Similar experiments are being done using the vMIPs genes of HHV-8 isolated from KS patients.
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