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Fundamental research about the ketone body therapy for Alzheimer's disearse.

Research Project

Project/Area Number 11670625
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Neurology
Research InstitutionTottori University

Principal Investigator

NAKASHIMA Kenji  Tottori University, Professor, 医学部, 教授 (70144673)

Co-Investigator(Kenkyū-buntansha) KASHIWAYA Yoshihiro  Tottori University, Professor, 医学部・附属病院, 医員
TAKESHIMA Takao  Tottori University, Professor, 医学部, 講師 (20206973)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,600,000 (Direct Cost: ¥1,600,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
KeywordsKetone Body / Amyloid βpeptide / Primary Hippocampal culture / Alzheimer's model / Energy crisis / マイクロアイランド培養法 / 初代海馬神経培養
Research Abstract

We have created the Amyloid beta protein toxicity model using rat primary hippocampal culture. Hippocampal cells are taken from 18 day fetal SD rats, which were cultured with absolute serum free medium. We have succeeded low density culture more that 7 days, with well neurites grouth and without increasing glial cells. Exposure of 5μ amyloicl s 1-42 peptide (Aβl-42) decreased cell number and neurite number and length in comparison to control. Addition of ketones to cells exposed to Aβ1-42 doubled the survived cell number and increased cell size and neurite outgrowth compared with cells exposed to Aβ1-42. Even we have developed the septum culture as well as hippocampal culture, the immunostaining with cholinergic neurons is unstable to have a quantitative data at this point.
It is known that Aβ1-42 is increased up to 6 fold in brains from patients with Alzheimer's disease and that Aβ1-42 inactivates the pyruvate dehydrogenase (PDH) complex in septal neurons by activating the protein kinase. D-(-)-β-hydroxybutyrate (DBHB) is a normal substrate in brain, with provides Acetyl CoA to TCA cycle directly with bypassing PDH complex, and also produce the reducing agent NADH.It was thought that DBHB could rescue hippocampal neurons from the toxicity of Aβ1-42 with mechanism mentioned above. These data, which resulted that ketone bodies provide a neuroprotective effect against with increased levels of Aβ1-42, suggest that ketone body could be a potential candidate for use in the treatment of Alzheimer's disease and other neurological diseases associated with energy crisis.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (2 results)

All Other

All Publications (2 results)

  • [Publications] Kashiwaya,Y.: "D-β-Hydroxybutyrate Protects Neurons in Models of Alzheimer's and Parkinson's Disease"Proceedings of the National Acaderny of Sciences of the United States of America. 97巻・10号. 5440-5444 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kashiwaya, Y.: "D-β-Hydroxybutyrate Protects Neurons in Models of Alzheimer's and Parkinson's Disease"Proc.Nat.Acad.Sci.of the USA. Vol.97. 5440-5444 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary

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Published: 1999-04-01   Modified: 2016-04-21  

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