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MOLECULAR AND PHYSIOLOGOCAL MECHNISM OF VASCULAR INFLAMMATION AND REMODELING AFTER VASCULAR INJURY

Research Project

Project/Area Number 11670666
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionSHINSHU UNIVERSITY SCHOOL OF MEDICINE

Principal Investigator

IMAMURA Hiroshi (2000)  SHINSHU UNIVERSITY SCHOOL OF MEDICINE, LECTURER, 医学部, 講師 (60283264)

渡辺 徳 (1999)  信州大学, 医学部・附属病院, 助手 (50303467)

Co-Investigator(Kenkyū-buntansha) ISOBE Mitsuaki  TOKYO MEDICAL AND DENTAL UNIVERSITY, ASSOCIATE PROFESSOR, 医学部, 助教授 (80176263)
WATANBE Noboru  SHINSHU UNIVERSITY SCHOOL OF MEDICINE, ASSISTANT PROFESSOR, 医学部・附属病院, 助手 (50303467)
UCHIKAWA Shin-ichiro  SHINSHU UNIVERSITY SCHOOL OF MEDICINE, ASSISTATNT PROFESSOR, 医学部・附属病院, 助手 (50324265)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,300,000 (Direct Cost: ¥2,300,000)
KeywordsP-selectin / leukocyte / platelet / restenosis / remodeling / vascular tone / 血管反応性 / 血管平滑筋 / 内膜障害 / リモデリング / 動脈硬化 / 血管傷害 / 血管リモデリング / 平滑筋細胞
Research Abstract

A.Roles of P-Selectin in Inflammation, Neointimal Formation, and Vascular Remodeling after vascular injury
Using a rat balloon catheter-induced injury model, we examined the role of P-selectin in vascular injury processes. In the acute phase after the injury, P-selectin was intensely expressed on both activated platelets covering the denuded segment and endothelial cells of the inflamed adventitial small vessels. Treatment with an anti-P-selectin monoclonal antibody (MAb) for 8 consecutive days significantly inhibited neointimal formation at day 14, and this effect persisted at day 56, as compared with the control group. Vascular shrinking accompanying adventitial fibrosis was also attenuated at day 56. Accumulation of CD45-positive leukocytes in the developing neointima, media and adventitia at day 8 was significantly inhibited by treatment with the anti-P-selectin MAb. Scanning electron microscopy demonstrated that anti-P-selectin treatment resulted in a less thrombogenic surface of t … More he arterial intima, which featured a pseudoendothelial appearance at day 14 after injury. These results suggest that inhibition of P-selectin-mediated leukocyte recruitment prevents the development of neointimal formation, adventitial inflammation, and vascular shrinking and promotes pseudoendothelialization by luminal smooth muscle cells. This treatment thus beneficially affects vascular remodeling after balloon injury in rat.
B.Arterial Tone and Responses to Vasoactive Substances After Balloon Injury
To evaluate vascular tone and response to vasoactive substances of thickening arterial wall after vascular injury, normal and balloon injured rat carotid arteries were isolated and change in circumferential wall tension was measured. Basic vascular tone of balloon injured artery after high potassium treatment was diminished. Typical vaso-constrictive substances, norepinephrine, phenylephrine, prostaglandin (PG) F2-alpha, and Serotonin (5HT) induced increases in vascular tension were attenuated, but relative responses compared with high potassium induced contraction were not changed after balloon injury. Relaxation induced by sodium nitroprusside was diminished, but relative responses were not changed. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (14 results)

All Other

All Publications (14 results)

  • [Publications] Hayashi S,Watanabe N, et al: "Roles of P-Selectin in Inflammation, Neointimal Formation, and Vascular Remodeling in Balloon Injured Rat Carotid Arteries."Circulation. 102. 1710-1717 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Cioca PD,Watanabe N et al: "Apoptosis of peripheral blood lymphocytes is induced by cathecholamines."Japanese Heart Journal. 41. 385-398 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kitabayashi H,Isobe M,Watanabe N, et al: "ETY720 Prevents Development of Experimental Autoimmune Myocarditis through Reduction of Circulating Lymphocytes"Journal of Cardiovascular Pharmacology. 35. 410-416 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 渡辺徳,磯部光章: "動脈硬化・再狭窄病変におけるp53の役割"臨床免疫. 33. 588-593 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Imanura H,Momose T,Kitabayashi H, et al: "Pulmonary hypertension as a result of asymptomatic portosystemic shunt."Japanese Circulation Journal. 64. 471-473 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi, S, Watanabe, N, et al.: "Role of P-selectin in inflammation, neointimal formation, and vascular remodeling in balloon-injured rat carotid arteries."Circulation. 102. 1710-1717 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Cioca, D P, Watanabe, N, et al.: "Apoptosis of peripheral blood lymphocytes is induced by cathecholamines."Jpn.Heart J. 41. 385-398 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Imamura H, Momose T, Kitabayashi H, Takahashi W, Yazaki Y, Takenaka H, Isobe M, Sekiguchi M, Kubo K: "Pulmonary hypertension as a result of asymptomatic portosystemic shunt"Jpn Circ J. 64. 471-3 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kitabayashi, H, Isobe, M, Watanabe, N, et al.: "FTY720 Prevents Development of Experimental Autoimmune Myocarditis through Reduction of Circulating Lymphocytes."J Cardiovasc Pharmacol. 35. 410-416 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hayashi S,Watanabe.N, et al: "Roles of P-selectin in Inflammation, Neointimal Formation, and Vascular Remodelingin Balloon Injured Rat Carotid Arteries."Circulation. 102. 1710-1717 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Cioca PD,Watanabe N, et al: "Apoptosis of peripheral blood lymphocytes is induced by cathecholamines."Japanese Heart Journal. 41. 385-398 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kitabayashi H,Isobe M,Watanabe N, et al.: "FTY720 Prevents Development of Experimental Autoimmune Myocarditis through Reduction of Circulating Lymphocytes."Journal of Cardiovascular Pharmacology. 35. 410-416 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 渡辺徳,磯部光章: "動脈硬化,再狭窄病変におけるp53の役割"臨床免疫. 33. 588-593 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Imamura H,Momose T,Kitabayashi.H, et al: "Pulmonary hypertension as a result of asymptomatic portosystemic shunt."Japanese Circulation Journal. 64. 471-473 (2000)

    • Related Report
      2000 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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