• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Identification of Adventitial cells and Molecular Mechanisms of Phenotypic Modulation in the vasclar remodeling after bolloon injury

Research Project

Project/Area Number 11670678
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionKobe University

Principal Investigator

TANIGUCHI Takahiro  Kobe University, School of medicine, associate professor, 医学部・附属病院, 講師 (20263379)

Co-Investigator(Kenkyū-buntansha) KAWASHIMA Seinosuke  Kobe University, School of medicine, associate professor, 医学部, 助教授 (10177678)
ISHIKAWA Yuichi  Kobe University, School of medicine, professor, 医学部, 教授 (90159707)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2000: ¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
Keywordsrestenosis / angioplasty / vascular remodeling / adventitial cell / monocyte / macrophage / differentiation / carotid arteries
Research Abstract

Perctaneous transluminal coronary angioplasty and other vascular reconstructive procedure are effective treatments for ischemic heart disease. However, the incidense of postprocedural restenosis ranges from 30% to 50%. Therefore the mechanisms of restenosis should be elucidated and effective treatments for restenosis should be developed as soon as possible. AS one of the mechanisms of restenosis, it is thought that migration and proliferation of medial vascular smooth muscle cells and synthesis of extracellular matrix result in neointimal formation. Recently attention has focused on the role of adventitial cells in postprocedual restenosis. But little is known about their role, origin, and etc. Our object is to investigate their role and origin after balloon injury of rat carotid artery. At 2 days after injury, many proliferating cells labelled with BrdU were located around the adventitia lesion whereas the media demonstrated infrequent labelled cells or cell death. After that, labelle … More d cells were found to translocate to media and neointima lesion. These adventitial cells were expressing α-SM actin, SMemb, vimentin, ED1, Myf5 and MEF2 but no staining for MyoD and Myogenin. These migrating adventitial cells were ED1 positive ; ED2 positive cells never transmigrated into media and neointima. Therefore, these data suggest that adventitial myofibroblasts (positive for α-SM actin, SMemb and vimentin) were expressing muscle genes and may derive from hematogenous mononuclear leukocytes. To confirm this, we examined the cultured monocytes for production of muscle genes. Initially, almost all monocytes were positive for Myf5 and ED1 but were negative for MyoD, MEF2 and Myogenin. After 5 days in culture, these cells expressed MEF2 and some cells expressed α-SM actin and SMemb. These findings suggest that hematogenous mononuclear leukocytes can develop into myofibroblasts and contribute to neointimal formation and arterial remodelling after angioplasty. We would like to find the differentiation factors and their intracellular signal transduction of adventitial cells to elucidate the mechanism of restenosis and to develop effective treatments. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report

Research Products

(21 results)

All Other

All Publications (21 results)

  • [Publications] Satoru Kawasaki: "Chylomicron remnant induces apoptosis in vascular endothelial cells"Annals of the New York Academy of Science. 902. 336-341 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akira Nobusawa: "Glibenclamide inhibits accumulation of cholesteryl ester in THP-1 human macrophages"Jounal of Cardiobasclar Pharmacology. 36. 101-108 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ken Matsumoto: "Effects of hypoxia on cholesterol metabolism in human monocyte-derived macrophages"Life Science. 67. 2083-2091 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomosaburo Takahashi: "Participation of reactive oxygen intermediates in the angiotensin II-activated signaling pathways in vascular smooth muscle cells"Annals of the New York Academy of Science. 902. 283-287 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akihiro Takahashi: "Tranilast inhibits vascular smooth muscle cell growth and intimal hyperplasia by induction of p21 waf1/cip1/Sdi1 and p53"Circulation Research. 84. 543-550 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomosaburo Takahashi: "Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells"American Journal of Physiology. 276. H1927-H1934 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoru Kawasaki: "Chylomicron remnant induces apoptosis in vascular endothelial cells"Annals of the New York Academy of Science. 902. 336-341 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akira Nobusawa: "Glibenclamide inhibits accumulation of cholesteryl ester in THP-1 human macrophages"Jounal of Cardiobasclar Pharmacology. 36. 101-108 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ken Matsumoto: "Effects of hypoxia on cholesterol metabolism in human monocyte-derived macrophages"Life Science. 67. 2083-2091 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomosaburo Takahashi: "Participation of reactive oxygen intermediates in the angiotensin II-activated signaling pathways in vascular smooth muscle cells"Annals of the New York Academy of Science. 902. 283-287 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Akihiro Takahashi: "Tranilast inhibits vascular smooth muscle cell growth and intimal hyperplasia by induction of p21waf1/cip1/Sdi1 and p53"Circulation Research. 84. 543-550 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Tomosaburo Takahashi: "Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells"American Journal of Physiology. 276. H1927-H1934 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoru Kawasaki: "Chylomicron remnant induces apoptosis in vascular endothelial cells"Annals of the New York Academy of Science. 902. 336-341 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akira Nobusawa: "Glibenclamide inhibits accumulation of cholesteryl ester in THP-1 human macrophages"Jounal of Cardiobasclar Pharmacology. 36. 101-108 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ken Matsumoto: "Effects of hypoxia on cholesterol metabolism in human monocyte-derived macrophages"Life Science. 67. 2083-2091 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Tomosaburo Takahashi: "Participation of reactive oxygen intermediates in the angiotensin II-activated signaling pathways in vascular smooth muscle cells"Annals of the New York Academy of Science. 902. 283-287 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshio Fujioka: "The significance of acidic sugar chains of apolipoprotein B-100 in cellular metabolism of low density lipoproteins"Journal of Laboratory and Clinical Medicine. 136. 355-362 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Akihiro Takahashi: "Tranilast inhibits vascular smooth muscle cell growth and intimal hyperplasia by induction of p21waf1/cip1/Sdi1 and p53"Circulation Research. 84. 543-550 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tomosaburo Takahashi: "Activation of Akt/protein kinase B after stimulation with angiotensin II in vascular smooth muscle cells"American Journal of Physiology. 276. H1927-H1934 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Tomosaburo Takahashi: "Participation of reactive oxygen intermediates in the angiotensin II-activated signaling pathways in vascular smooth muscle cells"Annals of the New York Academy of Science. (in press). (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Satoru Kawasaki: "Chylomicron remnant induces apoptosis in bovine aortic endothelial cells"Annals of the New York Academy of Science. (in press). (2000)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-03-31   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi