| Project/Area Number |
11670693
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Circulatory organs internal medicine
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| Research Institution | Kumamoto University |
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Principal Investigator |
KUGIYAMA Kiyotaka Kumamoto University School of Medicine, Department of Cardiovascular Medicine, Assistant Professor., 医学部・附属病院, 講師 (00225129)
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| Co-Investigator(Kenkyū-buntansha) |
泰江 弘文 熊本大学, 医学部, 教授 (40174502)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥900,000 (Direct Cost: ¥900,000)
Fiscal Year 1999: ¥2,600,000 (Direct Cost: ¥2,600,000)
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| Keywords | Coronary artery spasm / Oxidative stress / Angina pectoris / Reduced glutathione / Oxidized Low-density lipoproteins |
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| Research Abstract |
We examined the effect of reduced glutathione (GSH), an important antioxidant which restores intracellular redox imbalance and prevents inactivation of endothelial-derived nitric oxide, on the abnormal vasomotor reactivity in spasm coronary arteries. Responses of epicardial diameter of the left coronary arteries to intracoronary infusion of acetylcholine (ACh, 50μg/min) were measured by quantitative coronary angiography before and during combined intracoronary infusion of GSH (50mg/min, 6 min) or saline as a placebo in 24 patients with coronary spastic angina (CSA) and in 28 control patients. All of the spasm coronary arteries showed constrictor response to ACh, while the control coronary arteries as a whole showed only minimal diameter changes to ACh. GSH infusion suppressed constrictor response of epicardial diameter to ACh in patients with CSA, while it had no significant effect in control subjects. Saline infusion did not have any effects. The results indicate that GSH attenuated constrictor response to ACh in epicardial coronary arteries of patients with CSA.GSH may have an important role in the regulation of coronary vasomotor function in patients with CSA.
Oxidized low-density lipoproteins (LDL) impair endothelium-dependent dilation and constrict arteries. This study examined possible relation of the circulating plasma levels of Ox-LDL to CSA.The plasma levels of Ox-LDL were measured by ELISA in 37 consecutive patients with CSA and normal coronary angiograms and in 79 consecutive control patients. The Ox-LDL levels in patients with CSA were significantly higher than those in controls. In multivariate analysis, higher levels of Ox-LDL were a risk factor for CSA independently of other traditional risk factors. The Ox-LDL levels had a significant and positive correlation with constrictor response of coronary arteries to the intracoronary acetylchnoline infusion. Thus, Ox-LDL may play a possible role in pathogenesis of coronary spasm.
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