Effect to angiogenesis due to implantation of autologous tissue and fibroblasts in ischemic heart

Research Project

Project/Area Number	11670712
Research Category	Grant-in-Aid for Scientific Research (C)
Allocation Type	Single-year Grants
Section	一般
Research Field	Circulatory organs internal medicine
Research Institution	Tokyo Women's Medical University
Principal Investigator	KUSUMOTO Miyako Tokyo Women's Medical University, Dept of Cardiology, Professor, 医学部, 教授 (90075284)
Co-Investigator(Kenkyū-buntansha)	TOMIZAWA Yasuko Tokyo Women's Medical University, Dept of Cardiovascular Surgery, Instructor, 医学部, 助手 (00159047)
Project Period (FY)	1999 – 2000
Project Status	Completed (Fiscal Year 2000)
Budget Amount *help	¥3,500,000 (Direct Cost: ¥3,500,000) Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000) Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywords	Autologous tissue / incubation / implantation / angiogenesis / ischemic heart disease / fibroblast / capillaries / bFGF / angrogenesis / 虚血部 / autologous / 組織 / REC / 家兎 / 末梢血管
Research Abstract	From outside the body, the rabbit ear chamber (REC) provides a unique system for viewing capillaries in vivo. Most chambers are made of transparent easy-reflect materials including glass, acrylic resin and mica. The quality of macroscopic pictures of capillaries in the REC has not been satisfactory. With an illuminator from behind, flare was common, and with a flash light, reflection occurred. To obtain clear macroscopic pictures of REC with good contrast and sharp edges without flare, we made a new system. We used a Nikon F4s with a Medical Nikkor on a Nikon Multi photo stand. In a black light box, a strobe, Nikon Macro Speed Light SB-21, and a Modeling lamp were loaded. A 1-cm diameter hole was made at the edge on the top of the light box. Filming was done without difficulty. Without anesthetizing or stressing the animal, pictures were taken for one or two minutes. Without treatment, angiogenesis was observed at day 7 and vasculature was complete at 4 weeks in the REC field. The quality of pictures was satisfactory with contrast and sharp edges without flare up to 6 months. Our results suggest that this system is excellent to obtain direct evidence of angiogenesis in vivo from immediately after at weekly intervals over a long period.