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A study on the mechanism of myocardial injury in myocarditis and dilated cardiomyopathy.

Research Project

Project/Area Number 11670714
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Circulatory organs internal medicine
Research InstitutionTokyo Women's Medical University

Principal Investigator

NISHIKAWA Toshio  Tokyo Women's Medical University, Associate Professor, 医学部, 助教授 (50120019)

Co-Investigator(Kenkyū-buntansha) ISHIYAMA Shigeru  Tokyo Women's Medical University, Assistant, 医学部, 助手 (20297542)
MASUDA Akihiro  Tokyo Women's Medical University, Associate Professor, 医学部, 助教授 (60209434)
KASAJIMA Takeshi  Tokyo Women's Medical University, Professor, 医学部, 教授 (30045653)
HIROE Michiaki  Tokyo Medical Dental University, Instructor, 医学部, 講師 (80101872)
Project Period (FY) 1999 – 2002
Project Status Completed (Fiscal Year 2002)
Budget Amount *help
¥2,400,000 (Direct Cost: ¥2,400,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 1999: ¥900,000 (Direct Cost: ¥900,000)
KeywordsDilated cardiomyopathy / chronic myocarditis / rat / tissue injury / infiltrated lymphocyte / apoptosis / Fas / Fas ligand system / 抗Fas抗体 / 抗Fas ligand抗体 / 病理組織学 / Fas-Fas ligand / Fas-Fas ligand抗体投与 / Apoptosis / 病因 / 脾臓 / メモリーT細胞
Research Abstract

It has generally been recognized that myocarditis may be implicated in the pathogenesis of dilated cardiomyopathy. However, the mechanism of myocardial damage in the chronic stage of myocarditis is still unclear. In this study myocarditis was induced in Lewis rats by injection of porcine cardiac myosin and rats were harvested on day 21 or 74 after the initial injection. Apoptotic cell death was confirmed by in situ nick end labeling assay and gel-electrophoresis for DNA fragmentation on day 21, but none was disclosed on day 74. The expression of Fas mRNA and protein was detected on day 21 in the myocardium. In addition, Fas ligand was detected in some infiltrating lymphocytes. Some rats were injected with anti-Fas antibody or anti-Fas ligand antibody, and subsequently, myocardial damage was less severe than that in untreated rats on day 74. These results suggested that inhibition of apoptotic phenomenon in the acute stage may be related to the reduction of myocardial damage in the chronic stage of myocarditis.

Report

(5 results)
  • 2002 Annual Research Report   Final Research Report Summary
  • 2001 Annual Research Report
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Shigeru Ishiyama: "Inhibitory effects of vesnarinone in the progression of myocardial damage in experimental autoimmune myocarditis in rats"Cardiovasc Research. 43. 389-397 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takashi Shimjo: "Nitric oxide induces apoptotic death of cardiomyocytes via a cyclic-GMP-dependent pathway"Exp Cell Res. 247. 38-47 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshio Nishikawa: "Programmed cell death in the myocardium of arrhythmogenic right ventricular cardiomyopathy in children and adults"Cardiovasc Pathol. 8. 185-189 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshio Nishikawa: "The role of superoxide and nitric oxide in the development of myocardial injury in rat myocarditis"Microsc Microanal. 6(Suppl 2). 488-489 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshio Nishikawa: "Lymphoid cells and dendritic cells in transplanted hearts : an immunohistochemical study of three cases"Dendritic Cells. 10. 31-35 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasunari Sakomura: "Expression of proxisome proliferator-activated receptor-γ in vascular smooth muscle cells in upregulated in Marfan syndrome"Circulation. 106(Suppl I). I259-I263 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Shigeru Ishiyama, Michiaki Hiroe, Toshio Nishikawa, et al.: "Inhibitory effects of vesnarinone in the progression of myocardial damage in experimental autoimmune myocarditis in rats."Cardiovasc Research. 43. 389-397 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Takashi Shimojo, Michiaki Hiroe, Shigeru Ishiyama, Hiroshi Ito, Toshio Nishikawa, Fumiaki Marumo: "Nitric oxide induces apoptotic death of cardiomyocytes via a cyclic-GMP-dependent pathway."Exp Cell Res. 247. 38-47 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshio Nishikawa, Shigeru Ishiyama, et al.: "Programmed cell death in the myocardium of arrhythmogenic right ventricular cardiomyopathy in children and adults."Cardiovasc Pathol. 8. 185-189 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshio Nishikawa, Shigeru Ishiyama, et al.: "The role of superoxide and nitric oxide in the development of myocardial injury in rat myocarditis."Microsc Microanal. 6(suppl 2). 488-489 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Toshio Nishikawa, Takeshi kasajima, Akihiro Masuda, et al.: "Lymphoid cells and dendritic cells in transplanted hearts : an immunohistochemical study of three cases."Dendritic Cells. 10. 31-35 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] "Expression of proxisome proliferator-activated receptor-g in vascular smooth muscle cells is upregulated in Marfan syndrome."Circulation. 106 (suppl I). I-259-I-263 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2002 Final Research Report Summary
  • [Publications] Yasunari Sakomura: "Expression of proxisome proliferator-activated receptor-gamma in vascular smooth muscle cells is upregulated in cystic medial degeneration of annuloaortic ectasia in Marfan syndrome"Circulation. 106(suppl I). I・259-I・263 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Yoko Ishihara: "Expression and mutation of p53 gene in the lung of mice intratracheal injected with crystalline silica"Cancer Letters. 177. 125-128 (2002)

    • Related Report
      2002 Annual Research Report
  • [Publications] Kyoko Imanaka-Yoshida: "Tenascin-C modulates adhesion of cardiomyocytes to extracellular matrix during tissue remodeling after myocardial infarction"Laboratory Investigation. 81(7). 1015-1024 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西川俊郎: "孤立性心筋緻密化障害-Isolated noncompaction of the ventricular myocardium"病理と臨床. 19(12). 1360-1361 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西川俊郎: "転移性心臓腫瘍"循環器科. 50(3). 250-255 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Toshio Nishikawa: "The role of superoxide and nitric oxide in the development of myocardial injury in rat myocarditis."Microscopy Microanalysis. 6(suppl 2). 488-489 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Jun-ichi Suzuki: "Antisense bcl-x oligonucleotide induces apoptosis and prevents arterial neointimal formation in murine cardiac allografts."Cardiovascular Research. 45. 783-787 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shinichiro Imamura: "Behavior of smooth muscle cells during arterial ductal closure at birth."Journal of Histochemistry Cytochemistry. 48(1). 35-44 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Takashi Shimojo: "Nitric oxide induces apoptotic death of cardiomyocytes via a cyxlic-GMP-dependent pethway"Exp Cell Res. 247. 38-47 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shigeru Ishiyama: "Inhibitory effects of vasnarinone in the progression of myocardial damage in experimental autoimmune myocarditis in rats"Cardiovasc Research. 43. 389-397 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshio Nishikawa: "Ultrastructural features of the nyocardium of children with dilated cardio-myopathy"Heart Vessels. 14. 52-56 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshio Nishikawa: "Programmed cell death in myocardium of arrhythmogenic right ventricular cardiomyopathy in children and adults"Cardiovasc Pathol. 8. 185-189 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Toshio Nishikawa: "Histopathologic aspects of endomyocardial biopsy in pediatric patients with idiopathic ventricular tachycardia"Pediatrics Int. 41. 534-537 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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