| Project/Area Number |
11670748
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Fukui Medical University |
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Principal Investigator |
OHSHIMA Yusei Fukui Medical University, Faculty of Medicine, Pediatrics, Assistant, 医学部・小児科学, 助手 (40303391)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,000,000 (Direct Cost: ¥2,000,000)
Fiscal Year 2000: ¥100,000 (Direct Cost: ¥100,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
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| Keywords | Naive T cell / Th1 / Th2 / IL-13 / IL-12 / Cord blood / Allergy / Atopic dermatitis |
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| Research Abstract |
Background : Early intervention strategies in allergic disease are dependent on identification of newborns at high risk for later development of atopic disease.
Objective : In this cohort study of 106 neonates, we investigated whether CD4^+T cells production of cytokine and their responsiveness to IL-12 were associated with the subsequent development of atopic disease and whether a skewed cytokine production property was intrinsic to helper T cells.
Methods : To exclude the effects of contaminating cells, highly purified cord blood CD4^+ T cells were stimulated with anti-CD3 mAb and recombinant B7-2 molecule in the presence or absence of IL-12. Production of IL-13 and IFN-γ was determined by ELISA.Infants were assessed at 12 months for the development of atopic diseases.
Results : CD4^+T cells of neonates who manifested allergic symptoms (atopic group) produced higher levels of IL-13 compared with those of non-atopic group in both the presence and absence of IL-12. No significant difference was noted between the two groups with respect to IFN-γ production. There was no correlation between parental family history and cytokine production. Higher IL-13 production was observed in neonates with chronic eczema than those with short-term eczema.
Conclusion : Our data suggest that increased production of IL-13 by neonatal CD4^+T cells is a useful marker of newborns at high risk for subsequent development of atopic disease and that an intrinsic abnormality of CD4^+T cell is associated with the pathogeneses of atopic disease, especially atopic dermatitis.
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