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Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats

Research Project

Project/Area Number 11670751
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Pediatrics
Research InstitutionYamanashi Medical University

Principal Investigator

OHYAMA Kenji  Yamanashi Medical Mversity, Departait of Clinical Nursing, Professor, 医学部, 教授 (80051861)

Co-Investigator(Kenkyū-buntansha) NAKAGOMI Yoshiko  Yamanashi Medical Mversity, Department of Pediatrics, Assistant, 医学部, 助手 (20198055)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,800,000 (Direct Cost: ¥3,800,000)
Fiscal Year 2001: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥1,900,000 (Direct Cost: ¥1,900,000)
KeywordsGrowth Hormone / Spermatogenesis / Gyclophosphamide / Germ cell / Sperm / Dioxin / GH欠損ラット / ダイオキシン / Cyclophosphamide / 精子 / 下垂体摘出ラット
Research Abstract

Effects of Cyclophosphamide and Dioxin (TCDD) on spermatogenesis in rats
1. Effects of GH on recovery from testicular damage induced by Cyclophosphamide (CP) in GH-deficient rats. CP was intravenously administered at dose of 50 mg/kg for 2 days, and rat GH was subcutaneously administered at dose of 0.3 mg/kg every day for 2 and 4 weeks. The number of spermatocytes was decreased when they exposed to CP at type A spermatogonia. The.motility index of spermatozoa was also decreased by CP. In CP/GH group, the number of those cells was recovered and the damaged motility of spermatozoa was improved. Under GH-deficient condition, exogeneous GH can improve the testicular function damaged by CP.
2. Effect of very low dose of TCDD administered to maternal rats on ganadal function of neonatal rats. TCDD was subcutaneously administered at dose of 10 Rg/kg once a week to maternal rats (7, 14, 21 dpc, and 7, 14 days after birth). 21days after birth neonatal rats were subjected. All organs were normal by macroscopic examination, and testicular tissues were also microscopically normal. Expression of Cyp lAlspecifically induced by TCDD was markedly increased in neonatal rat liver. Inhibin B (marker of Sertoli cell function) expression in testis was normal.

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Yamano Y, Ohyama K: "A novel spermatogenesis-related factor-1 gene expressed in maturing testis"Biochem Biophys Res Commun. 289(4). 888-893 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka T, Ohyama K: "Growth-promoting effect of GH treatment at various doses in children with intrauterine growth retardation"Clin Pediatr Endocrinol. 10(suppl5). 15-23 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohyama K: "Molecular basis and clinical manifestation of McCune-Albright syndrome"Clin Pediatr Endocrinol. 9(1). 1-5 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogawa M, Ohyama K: "Prevalence of GH-1 gene deletion in patients with isolated growth hormone deficiency in Japan"Endocr J. 47(2). 157-162 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohyama K: "Growth hormone advances spermatogenesis in premature rats treated with gonadotropin-releasing hormone agonist"Endocr J. 46(4). 555-562 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamano Y, Ohyama K,: "vA novel spermatogenesis-related factor-1 gene expressed in maturing testis."Biochem Biophys Res Commun. 289. 888-893 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Tanaka T, Ohyama K,: "Growth-promoting effect of GH treatment at various doses in children with intrauterine growth retardation"Clin Pediatr Endocrinol. 10(suppl l5). 15-23 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohyama K: "Molecular Imsis and clinical manifestation of McCune-Albright syndrome"Clin Pediatr Endocrinol. 9 (1). 1-5 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ogawa M, Ohyama K: "Prevalence of GH-1 gene deletion in patients with isolated growth hormone deficiency in Japan"Endocr J. 47 (2). 157-162 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ohyama K: "Growth hormone advances spermatogenesis in .premature rats treated with gonadotropin-releasing hormone agonist"Endocr J. 46 (4). 555-562 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yamano Y, Ohyama K: "A novel spermatogenesis-related factor-1 gene expressed in maturing testis"Biochem Biophys Res Commun. 289(4). 888-893 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Tanaka T, Ohyama K: "Growth-promoting effect of GH treatment at various doses in children with intrauterine growth retardation"Clin Pediatr Endocrinol. 10(supp15). 15-23 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohyama K: "Molecular basis and clinical manifestation of McCune-Albright syndrome"Clin Pediatr Endocrinol. 9(1). 1-5 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ogawa M: "Prevalence of GH-1 gene deletion in patients with isolated growth hormone deficiency in Japan"Endocr J. 47(2). 157-162 (2000)

    • Related Report
      2001 Annual Research Report
  • [Publications] Ohyama K: "Molecular basis and clinical manifestation of McCune-Albright syndrome"Clin Pediatr Endocrinol. 9(1). 1-5 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ogawa M: "Prevalence of GH-1 gene deletion in patients with isolated growth hormone deficiency in Japan"Endocr J. 47(2). 157-162 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ohyama K: "Growth hormone advances srematogenesis in premature rats treated with gonadotropin-releasing hormone agonist"Endocr J. 46(4). 555-562 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ohyama K: "Growth hormone advances spermatogenesis in premature rats treated with gonadotropin-releasing hormone agonist"Endocr J. 46(4). 555-562 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohyama K: "Restoration of seminiferous tubular function after discontinuation of long-term gonadotropin-releasing hormone agonist in premature male rats"Endocr J. 44(4). 459-465 (1997)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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