The clarification of pathology of allergic Reactions to gelatin-containing vaccine.

• Project/Area Number: 11670771
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Single-year Grants
• Section: 一般
• Research Field: Pediatrics
• Research Institution: Sapporo Medical University
• Principal Investigator: TSUTSUMI Hiroyuki Department of Pediatrics, Sapporo Medical University School of Medicine, Assistant Professor, 医学部, 講師 (80217348)
• Project Period (FY): 1999 – 2000
• Project Status: Completed (Fiscal Year 2000)
• Budget Amount: ¥3,300,000 (Direct Cost: ¥3,300,000); Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000); Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
• Keywords: gelatin / gelatin allergy / Th1 / Th2 / IL-4 / IL-13 / IL-2 / IFN-γ

Research Abstract

We investigated the characteristics of T lymphocytes from patients allergic to gelatin-containing vaccine by secondary in vitro stimulation of circulating mononuclear cells with gelatin.
Patients with an immediate reaction all had gelatin-specific IgE antibody, whereas others did not. Lymphocytes from subjects with nonimmediate-type reactions generally expressed very weak or sometimes no IFN-γ, IL-2 , or IL-13 genes and especially no IL-4 gene. On the other hand, the lymphocytes of subjects with immediate-type reactions significantly expressed not only IL-4 and IL-13 but also IFN-γ and IL-2 mRNA.These suggest that both gelatin-specific Th2 and Th1 responses are involved in the pathogenesis of the immediate reaction to gelatin. The gelatin-specific IL-4 and/or IL-13 responses consistently observed in patients with an immediate reaction may be associated with the production of gelatin-specific IgE antibody.

Research Products

• [Publications] Ohsaki M,Tsutsumi H et al.: "The relevance of Th1 and Th2 cells in-"J Allergy Clin Immunol. 103. 276-281 (1999)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Kumagai T,Tsutsumi H et al.: "The lymphoprolife rative response to-"Clin Exp Allergy. 30. 1430-1435 (2000)
  • Description: 「研究成果報告書概要(和文)」より
• [Publications] Ohsaki M, Tsutsumi H, Kumagai T, Yamanaka T, Wataya Y, Furukawa H, Kojima H, Saito A, Yano S, Chiba S: "The relevance of Th1 and Th2 cells in immediate and nonimmediate reactions to gelatin-containing vaccine."J Allergy Clin Immunol. 103. 276-281 (1999)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Kumagai T, Nakayama T, Kamada M, Igarashi C, Yuri K, Furukawa H, Wagatsuma K, Tsutsumi H, Chiba S, Kojima H, Saito A, Okui T, Yano S: "The lymphoproliferative response to enzymatically digested gelatin in subjects with gelatin hypersensitivity."Clin Exp Allergy. 30. 1430-1435 (2000)
  • Description: 「研究成果報告書概要(欧文)」より
• [Publications] Ohsaki M,Tsutsumi H, et al: "The relevance of Th1 and Th2 cells-"J Allergy Cliu Immunol. 103. 276-281 (1999)
• [Publications] Kumafai T,Tsutsumi H et al: "The lymphoproliferative response to-"Clin Exp Allergy. 30. 1430-1435 (2000)
• [Publications] Ohsaki M, Tsutsumi H, et al: "The relevance of Th1 and Th2 cells in immediate〜"J Allergy Clin Immuocol. 103. 276-281 (1999)
• [Publications] Kumagai T, Tsutsumi H, et al: "The lymphoproliferative response to enzymatically"Clin Exp Allergy. (in press).