| Project/Area Number |
11670771
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | Sapporo Medical University |
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Principal Investigator |
TSUTSUMI Hiroyuki Department of Pediatrics, Sapporo Medical University School of Medicine, Assistant Professor, 医学部, 講師 (80217348)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,300,000 (Direct Cost: ¥3,300,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | gelatin / gelatin allergy / Th1 / Th2 / IL-4 / IL-13 / IL-2 / IFN-γ |
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| Research Abstract |
We investigated the characteristics of T lymphocytes from patients allergic to gelatin-containing vaccine by secondary in vitro stimulation of circulating mononuclear cells with gelatin.
Patients with an immediate reaction all had gelatin-specific IgE antibody, whereas others did not. Lymphocytes from subjects with nonimmediate-type reactions generally expressed very weak or sometimes no IFN-γ, IL-2 , or IL-13 genes and especially no IL-4 gene. On the other hand, the lymphocytes of subjects with immediate-type reactions significantly expressed not only IL-4 and IL-13 but also IFN-γ and IL-2 mRNA.These suggest that both gelatin-specific Th2 and Th1 responses are involved in the pathogenesis of the immediate reaction to gelatin. The gelatin-specific IL-4 and/or IL-13 responses consistently observed in patients with an immediate reaction may be associated with the production of gelatin-specific IgE antibody.
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