| Project/Area Number |
11670773
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | School of Health Sciences, Sapporo Medical University |
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Principal Investigator |
TACHI Nobutada School of Health Sciences, Sapporo Medical University., 保健医療学部, 助教授 (80136944)
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| Co-Investigator(Kenkyū-buntansha) |
KOZUKA Naoki School of Health Sciences, Sapporo Medical University, 保健医療学部, 助教授 (90225459)
NINOMIYA Tahafumi Department of Anatomy, Schhol of Medicine, Sapporo Medical University, 医学部, 講師 (80156140)
YAMASITA Tosihara Department of Dermatology, School of Medicine, Sapporo Medical University., 医学部, 講師 (50167706)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
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| Keywords | Mutated Po cDNA / constructed adenovirus vector / myelination / 変異Po cDNA / ラツト末梢神経 |
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| Research Abstract |
We identified new mutations of Po genes, including 433A-G, 543G-C, GGCA insertion between 603 to 604, in patients with Charcot-Marie-Tooth disease. We constructed Po cDNA clones containing these mutations by site-directed mutagenesis. These cDNA clones were inserted into expression adenovirus vector by Graham's method. Cultured rat peripheral nerve was peformed using dorsal root ganglions of fetal rat. Now constructed adenovirus vector containg mutated Po cDNA is infected in cultured rat peripheral nerve.
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