| Project/Area Number |
11670775
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Pediatrics
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| Research Institution | YOKOHAMA CITY UNIVERSITY |
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Principal Investigator |
IBE Masaaki SCHOOL OF MEDICINE, YOKOHAMA CITY UNIVERSITY ASSISTANT PROFESSOR, 医学部・附属病院, 講師 (00232588)
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| Co-Investigator(Kenkyū-buntansha) |
AIHARA Yuhko SCHOOL OF MEDICINE, YOKOHAMA CITY UNIVERSITY ASSISTANT PROFESSOR, 医学部・市民総合医療センター, 助教授 (50211686)
YOKOTA Shumpei SCHOOL OF MEDICINE, YOKOHAMA CITY UNIVERSITY PROFESSOR, 医学部, 教授 (10158363)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥2,800,000 (Direct Cost: ¥2,800,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
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| Keywords | HEPATITIS C VIRUS / HEPATITIS C / CYTOTOXIC T LYMPHOCYTE / EPITOPE / HLA / MOTHER-TO-INFANT TRANSSION |
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| Research Abstract |
In order to clarify the role of cytotoxic T lymphocytes (CTL) in hepatitis C virus (HCV) infection, an HLA-B35-restricted cytotoxic T cell epitope of HCV was identified using a strategy called reverse immunogenetics. Twenty-eight of 53 HCV peptides carrying two anchor residues were selected as HLA-B^*3501 binding peptides. These peptides were used to induce the specific cytotoxic T cells in peripheral blood lymphocytes from a patient with acute hepatitis C.Six HLA-B^*3501 binding peptides induced the peptide-specific CTL.One (HPNIEEVAL) of six peptides was confirmed as the epitope by the specific T cell clones. A sequence identical to the epitope was detected in isolates of the virus from the patient and a strong CTL response to this epitope was observed in the acute phase of hepatitis C but not in the recovery phase. The specific CTL for this epitope were not detected in peripheral blood lymphocytes from patients with chronic hepatitis C.Together these results suggest that the CTL specific for this epitope have an important role in the elimination of the virus in the patient.
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