| Project/Area Number |
11670816
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
|
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| Research Institution | Gunma University |
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Principal Investigator |
NEGISHI Izumi Gunma University School of Medicine, assistant professor, 医学部, 講師 (60292611)
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| Co-Investigator(Kenkyū-buntansha) |
OHNISHI Kazunori Gunma University School of Medicine, assistant professor, 医学部, 講師 (60176948)
ISHIKAWA Osamu Gunma University School of Medicine, professor, 医学部, 教授 (90168188)
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| Project Period (FY) |
1999 – 2001
|
| Project Status |
Completed (Fiscal Year 2001)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | filaggrin / knockout mouse / keratinocyte / apoptosis |
|---|
| Research Abstract |
It has been reported that filaggrin may be involved in the terminal differentiation (apoptosis) of keratinocytes. In order to study of keratinocyte differentiation in vivo, we are planning to generate filaggrin-deficient mice. Filaggrin is composed of approximately 320 amino acids and the unit of profilaggrin, which consists of multiple filaggrin-repeats. To generate filaggrin-deficient mice, we need to disrupt the region containing the initiation codon of the profilaggrin gene rather than the coding region of filaggrin. We succeeded to obtain a BAC clone containing the mouse profilaggrin gene with a human profilaggrin cDNA as a probe. We have subloned the important regions of this gene to design a targeting vector. At present, filaggrin^<+/-> ES cells are under construction.
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