Development of antigen-specific immunosuppressive therapy using dendritic cells.
| Project/Area Number |
11670822
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | Yamanashi Medical University |
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Principal Investigator |
KITAJIMA Toshiyuki Yamanashi Medical University, Dept. of Dermatology, Assistant Professor, 医学部, 講師 (40303408)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | Immunosuppression / Langerhans cell / Ultraviolet light / 樹状細胞 / XS106 |
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| Research Abstract |
Ultraviolet (UV) radiation is generally known to suppress immunological reaction. There are two proposal mechanisms regarding ultraviolet light-induced immunosuppression. One is mediated by keratunocytes which constitute skin. The other is mediated by langerhans cells (LC). The purpose of the study is making experimental animal models inducing immunotorelance using ultraviolet irradiated LC.We used XS106, new born skin-derived LC line as LC.Sunlamp (UVB) was used for radiation in the dose of 500 J/m^2. After LC were pulsed with antigen, UVB was irradiated and such LC were subcutaneously injected into mice. Delayed type hypersensitivity reaction against corresponding antigen was analyzed.
RESULT When UV irradiated LC were cocultured with T cells in vitro, cell death occurred in LC, and activation of T cells was inhibited. Mouse injected with UV-irradiated LC pulsed with antigen developed suppresed hypersensitivity reaction against the same antigen but not irrelevant ones. Therefore the suppresion was antigen-specific. We propose that UV-irradiated LC are useful to treat antigen-specific immune diseases.
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Report
(3 results)
Research Products
(5 results)
