| Project/Area Number |
11670823
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
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| Research Institution | NAGOYA UNIVERSITY |
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Principal Investigator |
MURO Yoshinao SCHOOL OF MEDICINE, NAGOYA UNIVERSITY, ASSOCIATE PROFESSOR, 医学部, 助教授 (80270990)
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| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥2,100,000 (Direct Cost: ¥2,100,000)
Fiscal Year 1999: ¥1,500,000 (Direct Cost: ¥1,500,000)
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| Keywords | anti-centromere antibody / CENP-A / CENP-B / CENP-C / antigen-driven / autoantibody / autoantigen / autoantigen / autoantibody / エピトープ |
|---|
| Research Abstract |
I tried to analyze human anti-centromere antibodies (ACA) longitudinally and to produce animal antibodies, which mimic human ACA concerning on autoepitopes, experimentally. In human patients, there were various differences of autoimmune phenomenon against between CENP-B and CENP-A/C.Although rabbits immunized with synthetic peptides, which corresponded to human CENP-A autoepitopes, produced anti-CENP-A antibodies which could be used for both immunoblotting and immunofluorescence studies, they could not produce anti-CENP-B or anti-CENP-C antibodies under the observation of more than six months. I am now considering a few candidates of immunogen to pruduce experimental antibodies mimicing human ACA.
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