| Project/Area Number |
11670830
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Dermatology
|
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| Research Institution | Kobe University |
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Principal Investigator |
OKA Masahiro Kobe University, School of Medicine, Research Associate, 医学部, 助手 (30252761)
|
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| Co-Investigator(Kenkyū-buntansha) |
小西 博昭 神戸大学, バイオシグナル研究センター, 助手 (40252811)
|
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| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 1999: ¥1,800,000 (Direct Cost: ¥1,800,000)
|
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| Keywords | protein kinase C / adenovirus vector / pyoderma gangrenosum / interleukin-8 / melanin / 黒色腫 / プロテインキナーゼC / 色素細胞 |
|---|
| Research Abstract |
We constructed recombinant adenovirus vectors for protein kinase C (PKC) isozymes (α, β, δ, ε, ζ), interleukin-8 (IL-8), constitutively active and dominant negative mutants of phosphatidylinositol 3-kinase (PI 3-kinase), and constitutively active mutant of Akt. Using these adenovirus vectors, we got several results as follows.
1. PKCα augments TPA-induced activation of phospholipase D in G361 melanoma cells which do not express PKCα.
2. IL-8 overexpression is present in pyoderma gangrenosum ulcers and leads to ulcer formation in human skin xenografts.
3. PI 3-kinase regulates melanogenesis by modulating the expression of tyrosinase, and activation of Akt is sufficient for suppression of melanin production in G361 melanoma cells.
4. Elimination of CD4+T cells enhances antitumor effect of locally secreted interleukin-12 on B16 mouse melanoma and induces vitiligo-likecoat color alteration.
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