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Gene therapy used by p53 of radioresistant tumor for enhancing radiosensitivity

Research Project

Project/Area Number 11670910
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Radiation science
Research InstitutionSaitama Medical School

Principal Investigator

TAKAHASHI Takeo  Saitama Medical School, Lecturer, 医学部, 講師 (70241883)

Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥2,700,000 (Direct Cost: ¥2,700,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,100,000 (Direct Cost: ¥1,100,000)
KeywordsNMT-1 / NMT-1R / apoptosis / p53 / radioseusitivity / gene therapy / p21 / bax
Research Abstract

Apoptosis is an important mechanism of cell death. P53 oncogene had a relationship with apoptosis. We have previously reported the establishment of two rat yolk sac tumor cell lines(NMT-1, NMT-1R), which were of the same origin and had different radiosensitivity. We reported that radioresistant cell line, NMT-1R, lossed function of p53 gene, and radiation-induced mutation of p53 decreased radiosensitivity. Apoptosis appeared in radiosensitive cell line, NMT-1, 24 h after irradiation in vitro. Expression of p53, p21, and bax proteins were elavated in NMT-1 cells after irradiation. However, there was no epression of p53, p21, and bax proteins in NMT-1. We assessed the status of p53 in two cell lines by PCR-SSCP. NMT-1 cells had wild type of p53 gene, however, mutant p53 was seen in NMT-1R sells. We tried injection of wild type of p53 gene to radioresistant NMT-1R cells. The result was that we failed enhancing radiosensitivity in radioresistant cell line

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (19 results)

All Other

All Publications (19 results)

  • [Publications] Akimoto T, Nonaka T, Ishikawa H, Sakurai H, Saito J, Takahashi T et al.: "Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro : possible involvement of inhibition of survival signal transduction pathways"International J Radiation Oncology Biol. Phys.. 50. 195-201 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hosono M, Machida K, Matsui T, Honda N, Takahashi T et al.: "Non-invasive quantitative monitoring of cerebral blood flow in aneurysmal subarachnoid haemorrhage with 99m-Tc-ECD"Nucleon Medicine Communications. 23. 5-11 (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hosono M, Machida K, Matsui T, Honda N, Takahashi T et al.: "Oligodendrogliona with cystic component infant"Computer Medical Imaging and Graphics. 25. 307-312 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto H, Takahashi T, Mitsuhashi N, Higuchi K, Niibe H: "Meditation of tower-associated antigen (CEA) expression of human lung caner cells by irradiation, either alone or in combination with Interferron-γ"Anticancer Research. 19. 307-312 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mitsuhashi N, Isuram MS, Sakurai H, Takahashi T et al.: "Is radiosensitive cell line cross-sensitive to heat? : Effect of heat on two rat yolk sac tumor cell lines with different radiosensitivity"Cancer Letters. 142. 195-200 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maebayashi, K., Mitsuhashi, N., Takahashi, T., Sakurai, H., Niibe, H: "p53 mutation decreased radiosensitivity in rat yolksac tumor cell lines"International J. Radiation Oncology Biol. Phys.. 44. 677-682 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Akimoto T, Nonaka T, Ishikawa H, Sakurai H, Takahashi T et al: "Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in Human esophageal cancer cell line in vitro :possible involvement of Inhabition of survival signal transduction"International J.Radiation Oncology Biol.Phys. 50. 195-201 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hosono M, Machida K, Matsui T, Honda N, Takahashi T et al: "Non-invasive quantitative monitoring of cerebral blood flow in Aneurysmal subarachnoid haemorrhage with 99mTc-ECD"nuclear Medicine Communications. 23. 5-11 (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Hosono M, Machida K, Matsui T, Honda N, Takahashi T et al: "Oligodendroglioma with cystic component in infant"Computerized Medical Imaging and Graphics. 25. 361-365 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Matsumoto H, Takahashi T, Mitsuhashi N, Higuchi K, Niibe H: "Modificaiton of tumor-associated antigen(CEA) expression of human Lung cancer cells by irradiation, either alone or in combination with Interferron-γ"Anticancer research. 19. 307-312 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Mitsyuhashi N, Isuram MS, Sakurai H, Takahashi T et al: "Is radiosensitive cell line cross-sensitive to heat? :Effect of heat on two Rat yolk sac tumor cell lines with different radiosensitivity"Cancer Letters. 142. 195-200 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Maebayashi K, Mitsuhashi N, Takahashi T, Sakurai H, Niibe H: "P53 mutation decreased radiosensitivitry in rat yolk sac tumor cell lines"International J, Radiation Oncology Biol.Phys.. 44. 677-682 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] T.Akimoto, T.Nonaka, H.Ishikawa, H.Sakurai, T.Takahashi et al.: "Genistein, a tyrosine kinase inhibitor, enhanced radiosensitivity in human esophageal cancer cell lines in vitro : possible involvement of inhibition of survival signal transduction pathways"Int. J. Radiation Oncology Biol. Phys.. 50・1. 195-201 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] M.Hosono, K.Machida, T.Matusi, N.Honda, T.Takahashi et al.: "Non-invasine quantitative manitoring of cerebral blood flow in aneulysmal subarachnoid haemorrhage with 99mTc-ECD"Nuclear Medicine Communications. 23. 5-11 (2002)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高橋健夫 他: "エビデンスに基づく放射線治療 インフォームド・コンセントの実践のために 8. 前立腺癌"臨床放射線. 46・8. 985-991 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 高橋健夫 他: "エビデンスに基づく放射線治療 インフォームド・コンセントの実践のために 10. 上咽頭癌"臨床放射線. 46・11. 1463-1468 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] Mitsuhashi N,Takahashi T et al: "Is radiosensitive cell line cross-sensitive to heat? : Effect of heat on two rat yolksac tumor cell lines with different radiosensitivity"Cancer Letters. 142. 195-200 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Matsumoto H, Takahashi T et al.: "Modification of Tumor-Associated Antigen (CEA) Expression of Human Lung Cancer cells by Irradiation, either Alare or in combination with Interferon-γ"Anticancer Research. 19. 307-312 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Maebayashi K, Mitsuhashi N, Takahashi T et al.: "p53 Mutation Decreased Radiosensitivity in Rat Yolk sac Tumor Cells Lines"Int. J. Radiation Oncology Biology・Physics. 44(3). 677-682 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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