| Budget Amount *help |
¥1,800,000 (Direct Cost: ¥1,800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Research Abstract |
The neuronal degeneration is characterized by neuronal loss and fibrillary gliosis, and reveals different grades of lesions in the regions of neurodegenerarive diseases such as pronressive supranuclear palsy (PSP), corticobasal degeneration, Alzheimer's disease, Pick's disease and spinocerebellar degeneration. For instances in PSP, severe lesions were in the subcortical neclei of globus pallidus, subthalamic nucleus and hypothalamus, and milder lesions in cerebral cortices.
To study the relationship between the neuronal degeneration and abnormal glial cytoskeletons, various stainings were performed, such as hematoxylin-eosin, Gallyas-Braak, Holzer, and immunostains including tau-2, ubiquitin, paired helical filament (PHF), bax, bcl-2 ans so on in the cases of above-mentioned diseases. In severe lesions of these diseases supposed to be highly degenerated portions, although there were different stainabilities in tau-2, PHF and ubiquitin, the similairty was detected in anti-bax stain, showing that cytoplasms and nuclear membranes stained strongly positive. In mild lesions there were no pisitive anti-bax findings and no correlations among argyrophil properties and these immunostains. In anti-bcl-2 staining, on the other hands, there were a few positive neuronal cells in the at-random regions in some neurodegenerative diseases.
In conclusion, the appearance of abnormal glial cytoskeletons, which were argyrophilic and tau-positive, were beyond the neuronal loss in cerebral cortices, and were suggested to have the relationship with programmed cell death. However, there were few evidences between apoptosis and glial cytoskeleton abnormality in this study.
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