| Project/Area Number |
11670953
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Psychiatric science
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| Research Institution | Miyazaki Medical College |
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Principal Investigator |
MATSUDA Kazunori Miyazaki Medical College, Research Associate, 医学部, 助手 (30311880)
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| Co-Investigator(Kenkyū-buntansha) |
林 要人 宮崎医科大学, 医学部, 助手 (20295223)
田代 謙一郎 九州保健福祉大学, 保健科学部, 教授 (00216954)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥1,200,000 (Direct Cost: ¥1,200,000)
Fiscal Year 2000: ¥400,000 (Direct Cost: ¥400,000)
Fiscal Year 1999: ¥800,000 (Direct Cost: ¥800,000)
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| Keywords | Laminin / Laminin peptide / Alzheimer's disease / Vascular dementia / ELISA / Cerebrospinal fluid / Diagnostic marker / Therapeutic agent / Therapeutic agent / ラミニンγ / wholeラミニン / ラミニンペプチド / 髄液 / アルツハイマー病(AD) / 脳血管性痴呆(VaD) / βアミロイド |
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| Research Abstract |
We developed a new ELISA system using antibodies against whole laminin-1, α5 chain, β1 chain, γ1 chain and laminin peptide (YFQRYLI). Using this ELISA system, we measured laminin levels in CSF obtained from Alzheimer type dementia (ATD), vascular dementia (VaD) and other dementia comparing with normal controls. ATD group showed a significantly lower level of γ1 chain, and the γ1 level of early -onset AD was significantly decreased with aging. On the other hand, late onset AD group showed a significantly higher level of laminin peptide (YFQRYLI) than early-onset AD group and normal controls. VaD group showed a significantly higer level of whole laminin-1 and β1 chain. These results suggest that laminin or its derivatives may correlate to pathogenesis of ATD and VaD, and that the preventing of laminin proteolysis may be an effective therapeutic method for preventing or slowing down the progression of ATD.Furthermore, the assay of CSF laminin for the diagnosis of ATD showed efficient diagnostic sensitivity (81%) and specificity (85%) by using the ratio of CSF tau to CSF whole laminin-1. These findings suggest that the assay of CSF laminin may be useful as a diagnostic biological marker of AD and VaD.
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