| Project/Area Number |
11670999
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Hematology
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| Research Institution | OKAYAMA UNIVERSITY |
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Principal Investigator |
IKEDA Kazuma OKAYAMA UNIVERSITY, MEDICAL SCHOOL HOSPITAL, LECTURER, 医学部・附属病院, 講師 (50176088)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENAKA Katsuto OKAYAMA UNIVERSITY, MEDICAL SCHOOL HOSPITAL, ASSISTANT, 医学部・附属病院, 助手 (30301295)
ISHIMARU Fumihiko OKAYAMA UNIVERSITY, MEDICAL SCHOOL HOSPITAL, ASSISTANT, 医学部・附属病院, 助手 (50284097)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥2,000,000 (Direct Cost: ¥2,000,000)
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| Keywords | Monocytes / macrophages / osteoclasts / ODF (RANKL) / LPS (endotoxin) / MCP-1 / IL-10 / UG3 / LPS(endotoxin) / MCP1 / IL-10 / IL-4 / IL-13 / M-CSF / osteoclast differentiation factor |
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| Research Abstract |
1. Differentiation of a monocytic line UG3 to osteocllasts
UG3 expressed osteoclast differentiating factor (ODF) receptor, RANK.Coculture with a stroma line ST2 transformed M-CSF-treated UG3 into tartarate-resistant acid phosphataase (TRAP)- and vitronectin receptor-positive multinucleated cells, which did not express calcitonin receptor, but fomred pits when cultured on bone slices. After incubation with ODF for 2 weeks, UG3 cells formed TRAP- and vitronectin receptor-positive multinucleated cells without calcitonin receptor mRNA expression.
2. Regulatory mechanisms of cytokine production by UG3 cells.
IL-4, IL-10 and IL-13 suppressed lipopolysaccharides (LPS)- induced production of IL-1β, IL-6, TNF α, IL-8, MCP-1, G-CSF and PGE2 by UG3 cells. IL-4 and IL-13 suppressed but IL-10 augmented MCP-1 production by unstimulated UG3 cells. IL-10 upregulated MCP-1 mRNA both transcriptionnally and posttranscriptionally without de novo protein synthesis. Binding of transcription factors to MCP-1 promotor was induced as follows : NFκB and Sp-1 by LPS, AP-1 and Sp-1 by TPA, and STAT1, STAT3 and Sp--1 by IL-10.
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