• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Cloning of a new gene derived from stromal cell related to suruiving and proliferation of pluripotent stem cells

Research Project

Project/Area Number 11671004
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Hematology
Research InstitutionKumamoto University

Principal Investigator

YONEMURA Yuji  Kumamoto University Hospital Blood Transfusion Service, Assistant, 医学部・附属病院, 助手 (60301371)

Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,500,000 (Direct Cost: ¥2,500,000)
Keywordspluripotent stem cell / stromal cell / skin fibroblast / hematopoiesis / 造血幹細胞
Research Abstract

We produced subtraction cDNA library between bone marrow stromal cells which support the hematopoiesis and skin fibroblasts which does not seem to support the hematopoiesis. 100 clones with insert exceeded 1.5 kb among these library were sequenced, and then the northern blot analysis of the unknown gene was done by using total RNA of bone marrow stromal cells and the skin fibroblasts. As the result, only 1 clone was high expression in the bone marrow stromal cells without completely observing the expression in the skin fibroblasts. The mRNA of this gene was big, and seemed to be between 8 and 9 kb. Although this gene has appeared in the peripheral blood lymphocytes, it has not completely appeared in the monocytes and macrophages. This gene has recognized the high expression also in the various leukemic cell lines. It was possible to get only 2 kb in the result of being the longest, though colony hybridization was done by using bone marrow stromal cell cDNA library for obtaining the ove … More rall length of this gene. In addition, long PCR was done using the arrangement of vector, however it was not possible that the base sequence of the 5' end was obtained. The arrangement of human genome has almost become clear by the progress of the Human Genome Project. It became clear that this gene had been carried on the 12th chromosome by computer analysis of this sequence. It is necessary to observe the expression in each hematopoietic lineage in the future. It seemed to be hematopoietic cell with the proliferation capability that has manifested this gene, and to maintain the hematopoiesis by the homomeric interaction with the bone marrow stromal cells. The screening will be carried out using the cDNA library of the random primer in the future, because the arrangement of the part that encodes the amine acid is not proven. In the meantime, the arrangement got by the Human Genome Project has been opened as an aggregate of the random arrangement to public, it is considered that it proves the order of this arrangement and picks out coding sequence out of genomic sequence using order and special software. Less

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (15 results)

All Other

All Publications (15 results)

  • [Publications] 福吉葉子,米村雄士: "末梢血幹細胞を適切な時期に効率良く採取する方法"日本検査血液学会誌. 2. 63-69 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shimomura T,Yonemura Y, et al.: "Thrombopoietin stimulates murine Lin, Sca-1+, c-Kit+, CD34- cells : Comparative study with SCF or IL-3."Int J Hematol. 71. 33-39 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohba T,Yonemura Y, et al.: "Aplastic anemia in pregnancy : Treatment with cyclosporine and granulocyte-colony stimulating factor."Acta Obstericia et Gynecologica Scandinavica. 78. 458-461 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 米村雄士: "「ニューミレニアムの輸血療法」-考えよう、輸血療法の必要性と安全性-トロソボポエチン"真興交易(株)医学出版部(印刷中). (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 米村雄士,松崎博充 他: "産科と婦人科"診断と治療社. 559-562 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Fukuyoshi Y, Yonemura Y, et al.: "Rational method of mobilization in peripheral blood stem cell transplantation."Journal of The Japanese Society for Laboratory Hematology. 2. 63-69 (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shimomura T, Yonemura Y, et al.: "Thrombopoietin stimulates murine Lin-, Sca-1+, c-Kit+, CD34- cells : Comparative study with SCF or IL-3."Int J Hematol. 71. 33-39 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohba T, Yonemura Y, et al.: "Aplastic anemia in pregnancy : Treatment with cyclosporine and granulocyte-colony stimulating factor."Acta Obstericia et Gynecologica Scandinavica. 78. 458-461 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shimomura T,Yonemura Y, et al.: "Thrombopoietin stimulates murine Lin-, Sca-1+, c-Kit+, CD34-cells : Comparative study with SCF or IL-3."Int J Hematol. 71. 33-39 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Ohba T,Yonemura Y, et al.: "Aplastic anemia in pregnancy : Treatment with cyclosporine and granulocyte-colony stimulating factor."Acta Obstericia et Gynecologica Scandinavica. 78. 458-461 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] 米村雄士: "「ニューミレニアムの輸血療法」-考えよう、輸血療法の必要性と安全性トロンボポエチン"真興交易(株)医学出版部(印刷中). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] 米村雄士,松崎博充 他: "産科と婦人科"診断と治療社. 559-562 (1999)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shimomura T,他7名: "Thrombopoietin stimulates murine lineage negative,Sca-1+,c-kit+,CD34-cells:Comparative study with stem cell factor or interleukin-3."Int.J.Hematol.. 65. 397-404 (2000)

    • Related Report
      1999 Annual Research Report
  • [Publications] Ohba T,他6名: "Treatment with cyclosporine and granulocyte-colony stimulating factor."Acta Obstericia et Gynecologica Scandinavica. 78. 458-461 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 米村雄士、松崎博充: "貧血"産科と婦人科. 66(4). 559-562 (1999)

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi