ANALYSIS OF MUCIN-TYPE CARBOHYDRATE AND LACTO-SERIES TYPEICHAIN EXPRESSION ON HEMATOPOIETIC PROGENITORS
| Project/Area Number |
11671013
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (C)
|
| Allocation Type | Single-year Grants |
|---|
| Section | 一般 |
|---|
| Research Field |
Hematology
|
|---|
| Research Institution | JICHI MEDICAL SCHOOL |
|---|
Principal Investigator |
MUROI Kazuo JICHI MEDICAL SCHOOL, DEPARTMENT OF MEDICINE, ASSOCIATE PROFESSOR, 医学部, 助教授 (50190939)
|
|---|
| Project Period (FY) |
1999 – 2000
|
| Project Status |
Completed (Fiscal Year 2000)
|
| Budget Amount *help |
¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
|
|---|
| Keywords | EARLY ERYTHROBLASTIC LEUKEMIA / SIALYL-TN / CELL LINE / CARBOHYDRATE / 赤芽球 / 白血病 |
|---|
| Research Abstract |
We encountered a patient with early erythroblastic leukemia. Flow cytometric, immunochemical and molecular studies were performed on leukemic blasts : The blasts expressed CD36 and CD71 but not lymphoid antigens, myeloid antigens, CD41 or glycophorin A.Analysis of carbohydrate antigens showed that the blasts expressed the sialyl-Tn antigen. Serum sialyl-Tn was markedly increased. Finally, an erythroid lineage was confirmed in the presence of alpha-globin messages in the blasts. A leukemic cell line, EEB, derived from leukemic cells of the patient was established. The phenotype, carbohydrate profile and globin expression of EEB cells were similar to those of leukemic cells from the patient. These results indicate that EEB cells are correspond to CFU-E.Erythropbietin did not induce the proliferation or differentiation of EEB cells. Western blot analysis showed that the molecular size of sialyl-Tn in EEB cells was identical to those in K562 cells, B-cell lymphoma cells and gastric cancer cells. Further studies are need to identify a role of sialyl-Tn on the proliferation, differentiation, adhesion and cell-to-cell interaction of EEB cells.
|
Report
(3 results)
Research Products
(3 results)
-
-
[Publications] MUROI K, TARUMOTO T, AKIOKA T, KIRITO K, NAGAI T, KIRITO K, NAGAI T, IZUMI T, NAKAMURA M, HATAKE K, HAKOMORI S, MIURA Y, OZAWA K.: "SIALYL-TN-AND NEURON-SPECIFIC ENOLASE-POSITIVE MINIMALLY DIFFERENTIATED ERYTHROLEUKEMIA."INTERNAL MEDICINE. 39(10). 843-846 (2000)
Description
「研究成果報告書概要(欧文)」より
Related Report
-
