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Study on mechanisms for inducing periventricular leucomalacia prior to delivery usig extrauterine incubation system of a goat fetus.

Research Project

Project/Area Number 11671075
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Embryonic/Neonatal medicine
Research InstitutionJuntendo university

Principal Investigator

YOSHIDA Koyo  Juntendo University, School of Medicine, Associate Professor, 医学部, 助教授 (90166950)

Co-Investigator(Kenkyū-buntansha) NISHINA Hidenori  Juntendo University, School of Medicine, Assistant Professor, 医学部, 講師 (70251270)
NIIJIMA Shinichi  Juntendo University, School of Medicine, Associate Professor, 医学部, 助教授 (20180557)
NAKAMURA Yasushi  Juntendo University, School of Medicine, Assistant Professor, 医学部, 講師 (70207926)
伊藤 茂  順天堂大学, 医学部, 助手 (20296867)
桑原 慶紀  順天堂大学, 医学部, 教授 (20010324)
Project Period (FY) 1999 – 2001
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2001: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
Keywordsexperimental perinatology / artificial womb / periventriclar leucoma ; acia / cerebral palsy / experimental model / fetus / ECMO / 脳性マヒ / variable deceleration / behavioral state
Research Abstract

To investigate the mechanisms for inducing periventricular leucomalacia (PVL) prior to delivery, we attempted to use the extrauterine incubation system on a goat fetus. It was hypothesized that consecutive ischemia and reperfusion to the brain of premature fetuses caused by transient umbilical venous compression could be a causative agent in the induction of PVL. Eleven goat fetuses of approximately 125 days gestational age (term 145 days) were incubated using extrauterine incubation system with arterio-venous extracorporeal membrane oxygenation (A-V ECMO). Acatheter connected to the umbilical vein was occluded completely (total occlusion in 5 cases) or partially (75% occlusion in 6 cases) for 15 seconds and then relieved from occlusion. Total volume of blood stored in a syringe during the occlusion was transfused over the next 45 seconds through the catheter. This procedure was repeated consecutively 6 times at intervals of 5 minutes. After the procedure, extrauterine incubation was c … More ontinued for up to 4 days. The goat fetus was then sacrificed, and the brain tissue was examined histopathologicaUy for the presence PVL lesions. During occlusion of the umbilical vein catheter, there was a significant decrease in fetal heart rate, fetalblood pressure and flow volume of the carotid artery as well as the amplitude of electrocorticogram. However, each of these recovered soon after relief from occlusion. There were no apparent differences between pre- and post- procedure blood gas examinations. The lesion of coagulation necrosis, which was recognized as the primary lesion of PVL, could be observed in the deep white matter surrounding the lateral ventricle in seven out of eleven goat fetuses. These results suggest that consecutive umbilical venous compression causing ischemia and reperfusion to the brain of premature fetuses may play an important role in the induction of PVL. As well, the extrauterine incubation system could provide an experimental animal model to investigate fetal pathology. Less

Report

(4 results)
  • 2001 Annual Research Report   Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report

Research Products

(13 results)

All Other

All Publications (13 results)

  • [Publications] 吉田 幸洋: "人工子宮の可能性"周産期医学. 31・1. 79-83 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 湯原 千治: "子宮外ヤギ胎仔保育システムを用いた脳室周囲白質軟化症(PVL)の発生に関する研究"日本新生児学会誌. 37・1. 36-41 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 西岡 暢子: "人工子宮の可能性"産婦人科の世界. 53・8. 129-133 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉田 幸洋: "人工胎盤の現状と将来"産科と婦人科. 67・12. 1777-1782 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉田 幸洋: "胎児PVL発生機序に関する研究-ヤギ胎仔子宮外保育システムを用いて-"日本新生児学会雑誌. 35・4. 700-704 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 米本 寿志: "臍帯動静脈A-V ECMOによる子宮外保育中のヤギ胎仔における心拍変動のスペクトル解析"日本産科婦人科学会雑誌. 53・12. 1819-1825 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉田 幸洋: "妊娠の生物学(分担) ヤギ胎仔を用いた子宮外保育実験"永井書店. 323-328 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] 吉田幸洋: "人工子宮の可能性"周産期医学. 31・1. 79-83 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 湯原千治: "子宮外ヤギ胎仔保育システムを用いた脳室周囲白質軟化症(PVL)の発生に関する研究"日本新生児学会誌. 37・1. 36-41 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 西岡暢子: "人工子宮の可能性"産婦人科の世界. 53・8. 129-133 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 米本寿志: "臍帯動静脈A-V ECMOによる子宮外保育中のヤギ胎仔における心拍変動のスペクトル解析"日本産科婦人科学会雑誌. 53・12. 1819-1825 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉田幸洋: "妊娠の生物学(分担) ヤギ胎仔を用いた子宮外保育実験"永井書店. 323-328 (2001)

    • Related Report
      2001 Annual Research Report
  • [Publications] 吉田幸洋: "胎児PVL発生機序に関する研究-ヤギ胎仔子宮外保育システムを用いて"日本新生児学会雑誌. 35・4. 700-704 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-03-31   Modified: 2016-04-21  

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