| Project/Area Number |
11671095
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Wakayama Medical University |
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Principal Investigator |
SAKAGUCHI Kazushige Wakayama Medical University Professor, 付置研究所, 教授 (60178548)
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| Co-Investigator(Kenkyū-buntansha) |
YOKOTE Hideyuki Wakayama Med Univ, Mol Med, Assistant Prof, 付置研究所, 助手 (60316122)
NAKAMURA Masayuki Wakayama Med Univ, Mol Med, Assistant Prof, 付置研究所, 助手 (40145917)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | parathyroid / tumor / oncogene / cDNA cloning / keratinocyte growth factor receptor / Yeast Two Hybrid / signal transduction / cDNA クローニング |
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| Research Abstract |
We have attempted systematical expression cloning of oncogenes from a cDNA library derived from a parathyroid adenoma, and functional analysis of these genes. An isoform of keratinocyte growth factor receptor, which has an alternatively spliced form of C-terminus, was isolated, and found to be expressed in parathyroid adenoma, but not in parathyroid cancer or hyperplasia. Two possibilities, the variant C terminus having functions of activating the signal transduction or the wild-type C terminus carrying the functions of inhibiting the signaling. To clarify these possibilities, we used the Yeast Two Hybrid system to clone the molecules that can bind to these C terminus portion of the keratinocyte growth factor receptor, which leads to the discovery of several molecules. We are now in the middle of characterizing the function of these clones.
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