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Study on the mechanism of stress response in the central nervous system.

Research Project

Project/Area Number 11671099
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Endocrinology
Research InstitutionNippon Medical School

Principal Investigator

SHIBASAK Tamotsu  Nippon Medical School, Faculty of Medicine, Professor, 医学部, 教授 (00147399)

Co-Investigator(Kenkyū-buntansha) OHATA Hisayuki  Nippon Medical School, Faculty of Medicine, Instructor, 医学部, 助手 (80256924)
ARAI Keiko  Nippon Medical School, Faculty of Medicine, Assistant Professor, 医学部, 講師 (60277118)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
Keywordscorticotropin releasing factor / stress / tail pinch / urocortin / food intake / conditioned fear / paraventricular nucleus of the hypothalamus / noradrenaline / corticotropin-releasing factor / CRF受容体 / CRF受容体拮抗薬 / 視床下部 / ストレス反応 / 覚醒
Research Abstract

One minute-tail pinch increased food intake in 30 min, and the effect was blocked by corticotropin-releasing factor (CRF) receptor type 1 (CRFR1) selective antagonist. Intracerebroventricular (icv) injection of CRF Increased and decreased food intake at doses of 2 ng and 1 μg, respectively. These results suggest that slight release of CRF is involved in tail pinch-induced food intake. In addition, we found that dopamine and opioid systems also participate in tail pinch-inducedfood intake.
Urocortin inhibited food intake in rats when it was microinjected to the ventromedial nucleus of the hypothalamus (VMH), and the effect was not affected by CRFR1 antagonist. The microinjection of anti-urocortin γ-globulin to the bilateral VMH increased food intake. Therefore, it is suggested that urocortin in the VMH has an inhibitory effect on food intake, and that the effect is not mediated by CRFR1.
Conditioned fear increased noradrenaline release in the paraventricular nucleus (PVN) of hypothalamus, and the change was blocked by CRFR1. It is therefore concluded that CRFR1 participates in conditioned fear-induced noradrenaline release in the PVN.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (25 results)

All Other

All Publications (25 results)

  • [Publications] Hotta M,Shibasaki T, et al: "Corticotropin-releasing factor receptor type 1 mediates emotional stress-induced inhibition of food intake and behavioral changes in rats."Brain Res. 823. 221-225 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Makino S,Shibasaki T, et al: "Psychological stress increased corticotropin-releasing hormone mRNA and content in the central nucleus of the amygdala but not in the hypothalamic paraventricular nucleus in the rats."Brain Res. 850. 136-143 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohata H,Suzuki K,Oki Y,Shibasaki T: "Urocortin in the ventromedial hypothalamic nucleus acts as an inhibitor of feeding behavior in rats."Brain Res. 861. 1-7 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Otagiri A,Wakabayashi I,Shibasaki T: "Selective corticotropin-releasing factor type 1 receptor antagonist blocks conditioned fear-induced release of noradrenaline in the hypothalamic paraventricular nucleus of rats."J Neuroendocrinol. 12. 1022-1026 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hotta M, Shibasaki T, et al: "Corticotropin-releasing factor receptor type 1 mediates emotional stress-induced inhibition of food intake and behavioral changes in rats."Brain Res. 823. 221-225 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Makino S, Shibasaki T, et al: "Psychological stress increased corticotropin-releasing hormone mRNA and content in the central nucleus of the amygdala but not in the hypothalamic paraventricular nucleus in the rats."Brain Res. 850. 143-146 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohata H, Suzuki K, Oki Y, Shibasaki T: "Urocortin in the ventromedial hypothalamic nucleus acts as an inhibitor of feeding behavior in rats."Brain Res. 861. 1-7 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Otagiri A, Wakabayashi I, Shibasaki T: "Selective corticotropin-releasing factor type 1 receptor antagonist blocks conditioned fear-induced release of noradrenaline in the hypothalamic paraventricular nucleus of rats."J Neuroendocrinol. 12. 1022-1026 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Shinozawa I, Inokuchi K, Wakabayashi I, Dan K.: "Disturbed expression of the anti-apoptosis gene, Survivin, and EPR-1 in hematological malignancies."Leukemia Research. 24. 965-970 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Watanabe A, Inokuchi K, Mizuki T, Yamaguchi H, Yokose N, Dan K.: "Repeated efficacy of all-trans-retinoic acid in an acute promyelocytic leukemia patient."American Journal Hematology. 65. 87 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Nakamura H,Inokuchi K, Hanawa H, Yamaguchi H, Tamura H, Tajika K, Dan K.: "A case of chronic myeloid leukemia with minor bcr-abl transcript following fluorouracil therapy for esophageal carcinoma"Annals of Hematology. 79. 396-401 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamaguchi H, Hashimoto M, Mizuki T, Nakamura H, Nakayama K, Nakamura K, Tajika K, Inokuchi K, Dan K.: "Skeletal muscle T-cell lymphoma following hemophagocytic syndrome."Japanese Journal Clinical Hematology. 41. 712-717 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Inokuchi K, Hamaguchi H, Taniwaki M, Yamaguchi H, Tanosaki S, Dan K.: "EstablishmentofacelllinewithAML1-MTG8, p53 and p73 abnormalities from acute myelogenous leukemia."Genes Chromosome Cancer. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamaguchi H, Inokuchi K, Tarusawa M, Dan K.: "Loss of bcl-xs expression is an importance prognostic factor in acute myeloid leukemia."Leukemia. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yamaguchi H, Inokuchi K, Sakuma Y , Dan K.: "Mutation of the p5l/p63 gene is associated with blastic crisis in chronic myelogenous leukemia."Leukemia. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Inokuchi K, YamaguchiH, Tarusawa M, Futaki M, Hanawa H, Tanosaki S, Dan K.: "Abnormality of c-kit oncoprotein in certain patients with chronic myelogenous leukemia-potential clinical significance."Leukemia.

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Inokuchi K, YamaguchiH, Hanawa H, Tanosaki S, Nakamura K, Tarusawa M, Miyake K, Shimada T, Dan K.: "Loss of DCC gene expression is of prognostic importance in acute myelogenous leukemia."Journal Clinical Oncology. (in press).

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Hisayuki Ohata: "Urocortin in the ventromedial hypothalamic nucleus acts as an inhibitor of feeding behavior in rats."Brain Research. 861・1. 1-7 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] A.Otagiri: "Selective corlicotropin-releasing factor type 1 receptor antagonist blocks conditioned fear-induced release of noradrenaline in the hypothalamic paraventricular nucleus of rats."Journal of Neuroendocrinology. 12・10. 1022-1026 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Shinya Ishii: "Response of leptin mRNA to 24-h food deprivation and refeeding is influenced by age in rats"Regulatory Peptides. 92・1-3. 45-50 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yujin Shuto: "Generation of polygonal antiserum against the growth hormone secretagogue recepcon (GHS-R) Evidence that the GHS-R exists in the hypothalamus, Pituitar and stomach of rats."Life Sciences. 68・9. 991-996 (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Keiko Arai: "Nicotine infusion alters leptin and uncoupling protein I mRNA expression in adipose tissues of rats"American Journal of Physiology. (in press). (2001)

    • Related Report
      2000 Annual Research Report
  • [Publications] Mari Hotta: "Corticotropin-releasing factor receptor type1 mediates emotional stress-induced inhibition of food intake and behavioral changes in rats"Brain Research. 823・1〜2. 221-225 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Shinya Makino: "Psychological stress increased corticotropin-releasing hormone mRNA and content in the nucleus of the amygdala but not in the hypothalamic paraventricular nucleus in the rat"Brain Research. 850・1〜2. 136-143 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] T. Fujioka: "The effects of prenatal stress on the development of hypothalamic paraventricular neurons in fetal rats"Neuroscience. 92・3. 1079-1088 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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