| Project/Area Number |
11671121
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Metabolomics
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| Research Institution | Kagawa Medical University |
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Principal Investigator |
TAMINATO Tomohiko Kagawa Medical University, Dept. of Laboratory Medicine, Professor, 医学部, 教授 (90107954)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,300,000 (Direct Cost: ¥1,300,000)
Fiscal Year 1999: ¥2,200,000 (Direct Cost: ¥2,200,000)
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| Keywords | CD38 / AntiCD38 antibody / Diabetes mellitus / Insulin secretion / ADP-ribosyl cyclase / Cyclic ADP-ribose |
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| Research Abstract |
The present study attempted to clarify the prevalence and significance of anti-CD38 antibody, which can be detected in sera from diabetic patients. Sera were collected from 184 type 2 diabetics. An ElA system was developed to detect anti-CD38 antibody using Keyhole Limpet Hemocyanin (KLH) -conjugated CD38 fragment peptides, 287-297 (N-CVKNPEDSSCT-C (Ag-1) and 241-255 (N-CSNNPVSVFWKTVSR-C (Ag-2), as antigens.
Among 184 diabetics, anti-CD38 antibody was detected in 43 patients. Most of antibody positive sera reacted with Ag-1 and a part of those reacted with Ag-2. The clinical characteristics of antibody-positive patients were short-duration of diabetes, higher blood glucose level, less white blood cell counts, and receiving diet or oral hypoglycemic agents, rather than insulin. Antibody-positive patients were shown to have relatively higher level of serum and urinary C-peptide than type 1 diabetics, suggesting modest potential reservation of insulin secretion. Anti-CD38 antibody was not concordant with anti-GAD or anti-microsomal antibodies in those patients. Pretreatment of partially purified lgG from the antibody positive sera inhibited D-glucose-induced insulin release from isolated rat islets.
These results suggest that anti-CD38 antibody has a significant diagnostic and prognostic values in the diagnosis of worsening of type 2 diabetes or progression to type 1 diabetes.
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