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The regulation of insulin targeted cell proliferation and differentiation in the insulin resistance ; the role of expression of glucose transporters

Research Project

Project/Area Number 11671132
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Metabolomics
Research InstitutionYokohama City University

Principal Investigator

SATOH Shinobu  University Hospital, Yokohama City University Assistant Professor, 医学部・附属病院, 講師 (80244424)

Co-Investigator(Kenkyū-buntansha) SEKIHARA Hisahiko  School of Medicine, Yokohama City University Professor, 医学部, 教授 (80126094)
ITO Takaaki  School of Medicine, Yokohama City University Associate Professor, 医学部, 助教授 (70168392)
NOGUCHI Yoshikazu  School of Medicine, Yokohama City University Assistant Professor, 医学部, 講師 (50180724)
CUSHMAN Samuel w.  National Institutes of Health, Chief
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Keywordsinsulin resistance / adipocyte / cell differentiation / cell proliferation / glucose transporter / GLUT1 / PPAR / apoptosis / 細胞分化増殖 / 膵β細胞 / IRS / GLUT
Research Abstract

We studied the regulation of insulin targeted cell proliferation and differentiation in the insulin resistance ; the role of expression of glucose transporter. To investigate the role of IRS-1 in endocrine pancreatic function, we have performed in vivo experiments of islet function in mice with knockout mutations in Insulin Receptor Substrate (IRS)-1 (IRS-1-/-), as well as in vitro studies on isolated islets. We found that immunostaining for GLUT2 was remarkably reduced in the beta-cells of IRS-1-/- islets. In pancreatic beta-cells, two types of granules can be distinguished by electron microscopy : mature granules with a dense core and immature granules with lightly stained content. The proportion of light granules in IRS-1-/- islets was increased and that of dark granules decreased in comparison with wild-type islets. These studies indicate that IRS-1 deficiency leads to reduced GLUT2 expression and insulin response to glucose. Thus, IRS-1 plays unique roles in beta-cell development and function. Heterozygous PPAR gamma-deficient mice were protected from the development of insulin resistance due to adipocyte hypertrophy under a high-fat diet. These phenotypes were abrogated by PPAR gamma agonist treatment. Heterozygous PPAR gamma-deficient mice showed overexpression and hypersecretion of leptin despite the smaller size of adipocytes and decreased fat mass, which may explain these phenotypes at least in part. This study reveals a hitherto unpredicted role for PPAR gamma in high-fat diet-induced obesity due to adipocyte hypertrophy and insulin resistance, which requires both alleles of PPAR gamma.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (24 results)

All Other

All Publications (24 results)

  • [Publications] Takaki Yoshikawa: "Inhibition ofIRS-1 phosphorylation and the altterations of GLUT4 in isolated adipocytes from cachectic tumor-bearing rats"Biochemical and Biophysical Research Communications. 256・3. 676-681 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naoto Kubota: "PPAR gamma mediates high-fat diet-fat diet-induced adipocyte hypertrophy and insulin resistance"Molecular Cell. 4・4. 597-609 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazutaka Aoki: "Dehydroepiandrosterone suppresses the elevated hepatic glucose-6-phosphatase and fructose-1, 6-bisphosphatase activities in C57BL/Ksj-db/db mice : comparison with troglitazone."Diabetes. 48・8. 1579-1585 (1999)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshikazu Noguchi: "Suppression of facilitative glucose transporter 1 mRNA can suppress tumor growth."Cancer Letter. 154・2. 175-182 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoshi Ito: "Human rhabdomyosarcoma cells retain insulin-regulated glucose transport activity through glucose transporter 1."Arch Biochem Biophys.. 373・1. 72-82 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naoto Kubota: "Disruption of insulin receptor substrate 2 causes type 2 diabetes because of liver insulin resistance and lack of compensatory beta-cell hyperplasia."Diabetes. 48・8. 1880-1889 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaki Yoshikawa ; Shinobu Satoh: "Inhibition of IRS-1 phosphorylation and thealtterations of GLUT4 in isolated adipocytes from cachectic tumor-bearing rats."Biochemical and Biophysical Research Communications. 256 3. 676-681 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naoto Kubota et al: "PPAR gamma mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance."Mol Cell. 4(4). 597-609 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazutaka Aoki et al: "Dehydroepiandrosterone suppresses the elevated hepatic glucose-6-phosphatase and fructose-1, 6-bisphosphatase activities in C57BL/Ksj-db/db mice : comparison with troglitazone."Diabetes. 48(8). 1579-1585 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takaaki Ito T ; Shinobu Satoh: "Glucose transporter expression in developing fetal lungs and lung neoplasms."Histology and Histopathology.. 14(3). 895-904 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yoshikazu Noguchi ; Shinobu Satoh: "Suppression of facilitative glucose transporter 1 mRNA can suppress tumor growth."Cancer Lett.. 154(2). 175-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoshi Ito et al: "Human rhabdomyosarcoma cells retain insulin-regulated glucose transport activity through glucose transporter 1."Arch Biochem Biophys.. 373(1). 72-82 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Naoto Kubota et al: "Disruption of insulin receptor substrate 2 causes type 2 diabetes because of liver insulin resistance and lack of compensatory beta-cell hyperplasia."Diabetes.. 49(11). 1880-1889 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Kazutaka Aoki et al: "Dehydroepiandreosterone suppresses ewlevated hepatic glucose・-phosphatase mRNA level in C57BL/KsJ-db/db mice. Comparison with troglitazone"Endocine J.. 47869. 799-804 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Satoshi Ito: "Human rhabdomyosarcoma cells retain insulin-regulated glucose transport activity through glucose transporter 1."Arch Biochem Biophys. 373・1. 72-82 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshikazu Noguchi: "Spression of facilitative glucose transporter 1 mRNA in colon cancer was not regulated by k-ras."Cancer Letter. 154・2. 137-142 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Naoto Kubota: "Disruption if insulin receptor substrate 2 causes type 2 diabetes because of liver insulin resistance and lackof compensatory β-cell hyperplasia"Diabetes. 49・11. 1880-1889 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Kazutaka Aoki: "Dehydroepiandrosterone supresses elevated hepatic glucose-6-phosphatase mRNA level in C57BL/KsJ-db/db mice"Endocrine J. 47・6. 799-804 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Yoshikazu Noguchi: "Suppression of facilitative glucose transporter 1 mRNA can suppress tumor growth"Cancer Letter. (In press).

    • Related Report
      1999 Annual Research Report
  • [Publications] Naoto Kubota: "PPARγ mediates high-fat diet-induced adipocyte hypertrophy and insulin resistance"Molecular Cell. 4. 597-609 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yasuo Terauchi: "Increased insulin sensitivity and hypoglycemia in lacking the p85α subunit of phosphoinositide 3-kinase"Nature Genetics. 21・2. 230-235 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Kazutaka Aoki: "Dehvdroepiandrosterone Suppresses the Elevated Hepatic Glucose-6-Phosphatase And Fructose-1,6-Bisphosphatase Activities in C57BL/Ksj-db/db Mice"Diabetes. 48・8. 1579-1585 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takaaki Ito: "Glucose transporter expression in developing fetal lungs and lung neoplasms"Histology and Histopathology. 14. 895-904 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Yoshikazu Noguchi: "Expression of facilitative glucose transporters in gastric tumors"Hepato-Gastroenterology. 46. 2683-2689 (1999)

    • Related Report
      1999 Annual Research Report

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Published: 2000-04-01   Modified: 2016-04-21  

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