| Project/Area Number |
11671145
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | TOHOKU University |
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Principal Investigator |
HARADA Yuko Tohoku Univ., School of Medicine Hospital, Research Associate, 医学部・附属病院, 助手 (90292317)
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| Co-Investigator(Kenkyū-buntansha) |
SATOMI Susumu Tohoku Univ., Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (00154120)
KUBOTA Kazuo Tohoku Univ., School of Medicine Hospital, Lecturer, 加齢医学研究所, 助教授 (40161674)
OHUCHI Noriaki Tohoku Univ., Graduate School of Medicine, Professor, 大学院・医学系研究科, 教授 (90203710)
ISHIDA Takanori Tohoku Univ., School of Medicine Hospital, Research Associate, 医学部・附属病院, 助手 (70323011)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,200,000 (Direct Cost: ¥3,200,000)
Fiscal Year 2000: ¥1,500,000 (Direct Cost: ¥1,500,000)
Fiscal Year 1999: ¥1,700,000 (Direct Cost: ¥1,700,000)
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| Keywords | ^<99m>Tc-MIBI / PET / Radiosensitivity / Angogenesis / P-ghyroprotein |
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| Research Abstract |
【Introduction】 Radiotherapy is a principal treatment in primary and metastatic breast cancer. The aim of this study was to investigate the difference of distribution of ^<99m>Tc-MIBI and deoxyglucose (DG) before and after irradiation in breast cancer models. We also investigated the correlation between microvessel density and ^<99m>Tc-MIBI uptake.【Materials and Methods】 Double-tracer macroautoradiography (ARG) images were prepared with ^<99m>Tc-MIBI and ^<14>C-DG in transplanted models using 3 mouse breast cancer cell lines (FM3A, MM48 and Ehrlich). The radiotherapeutic effects were monitored by measuring tumor burden, uptake and ARG finding using ^<99m>Tc-MIBI and ^<18>F-FDG.Microvessel density was quantified by immunohistochemical staining for CD34 and compared with ARG using ^<99m>Tc-MIBI.【Results】 Three tumors showed different growth rates and radiosensitivities, but ^<99m>Tc-MIBI uptake did not correlate significantly with the growth rates. Compared with ^<14>C-DG, ^<99m>Tc-MIBI accumulated more in cancer cells and less in infiltrating fibroblasts and macrophages in all tumor models. Microvessel density in tumor tissue correlated significantly with ^<99m>Tc-MIBI uptake on ARG.【Conclusion】 Accumulation of ^<99m>Tc-MIBI in cancer cells is preferential and can be used as a sensitive marker to examine the response of radiotherapy. Angiogenesis seems to enhance accumulation of ^<99m>Tc-MIBI in tumors. These characteristics may be favorable for tumor imaging using ^<99m>Tc-MIBI.
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