| Project/Area Number |
11671151
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
General surgery
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| Research Institution | The University of Tokyo |
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Principal Investigator |
SHINOZAKI Masaru (2000) the Univ. of Tokyo, Faculty of Medicine Assistant, 医学部・附属病院, 助手 (10312315)
韓 一秀 (1999) 東京大学, 医学部・附属病院, 助手 (60312310)
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| Co-Investigator(Kenkyū-buntansha) |
IKEDA Shigeo the Univ. of Tokyo, Faculty of Medicine medical staff, 医学部・附属病院, 医員
SAITO Hideaki the Univ. of Tokyo, Faculty of Medicine, Associate Professor, 医学部・附属病院, 助教授 (30134555)
小倉 信 東京大学, 医学部・附属病院, 助手 (50185568)
篠崎 大 東京大学, 医学部・附属病院, 助手 (10312315)
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥4,000,000 (Direct Cost: ¥4,000,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Keywords | surgical stress / Inflammatory bowel disease / Gut mucosal immunity / neutrophil / lymphocyte / cytokines / 全身防御反応 |
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| Research Abstract |
We investigated the influence of enterocytes stimulated with inflammatory cytokines on polymorphonuclear neutrophil (PMN) and lymphocyte chemotaxis.
Obtained results are as follows :
A human enterocyte cell line (FHs74 Int.) was grown in the lower wells of a two-compartment system with standard medium solution (DMEM) containing 4000 mM Gln (n=12). After confluent monolayer formation, the enterocytes were stimulated for 24 hours with various doses of TNF-alpha (0-100 ng/mL). Then, we performed PMN and lymphocyte chemotaxis assay. Confluent human umbilical vein endothelial cell monolayers grown on the inserts were overlaid with PMN or lymphocyte (5x106/mL) isolated from healthy volunteers. The number of migrated PMN or lymphocyte into the lower wells was counted after 45 minutes by flow cytometry.
Enterocytes stimulated with TNF-alpha upregulated PMN migration in α TNF-alpha-dose dependent manner.
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