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CLINICAL USE OF MOLECULAR EPIDEMIOLOGY FOR NOSOCOMIAL IMIPENEMRESISTANT PSEUDOMONAS AERUGINOSA INFECTIONS

Research Project

Project/Area Number 11671163
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field General surgery
Research InstitutionHIROSHIMA UNIVERSITY

Principal Investigator

YOKOYAMA Takashi  HIROSHIMA UNIVERSITY, MEDICAL HOSPITAL, PROFESSOR, 医学部・付属病院, 教授 (60034104)

Co-Investigator(Kenkyū-buntansha) TAKESUE Yoshio  HIROSHIMA UNIVERSITY, FACULTY OF MEDICINE, ASSISTANT, 医学部, 講師 (70197292)
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2000)
Budget Amount *help
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
KeywordsPsendomonas aeruginosa / nosocomial infection / molecular epidemiology / antibiotics resistance / carbapenem antibiotics / pulsed-field gel electrophoresis
Research Abstract

To elucidate the clinical use of genotyping for nosocomical infections of Pseudomonas aeruginosa (P.aeruginosa), we examined genotyping and serotyping of imipenem-resistant Pseudomonas aeruginosa (IRPA) isolated in our surgical ward. In 428 P.aeruginosa strains clinically isolated from the patients between 1987 and 1998, serotyping and genotyping were performed by the slideagglutination test and pulsed-field gel electrophoresis (PFGE), respectively. Suseeptibility ageinst imipenem was determined according to the NCCLS guidelines. Overall, the incidence of IRPA infection was 21.5%. The annual incidences of IRPA infections were particularly high in 1990, 1991 and 1994 (34-47%). Serologically, group-F strains were prevalent between 1990 and 1991, and group-B strains in 1994. In PFGE analysis, the identical pattern was shown in group-F strains, and three patterns were detected for group-B strains, suggesting that these outbreaks raged as nosocomial infections. By appropriate use of carbapenem antibiotics after 1995, the incidence of IRPA infections was reduced (14.6%) and neither serotyping nor PFGE analysis detected distinct outbreaks. However, computed dendrogram derived from digitized PFGE patterns revealed some sporadic nosocomial infections limited to "patient-to-patient". Although serotyping can be advocated for detecting outbreaks of nosocomial infections. PFGE may be useful for survey of "patient-to-patient" limited infection before outbreaks of nosocomial infections.

Report

(3 results)
  • 2000 Annual Research Report   Final Research Report Summary
  • 1999 Annual Research Report
  • Research Products

    (30 results)

All Other

All Publications (30 results)

  • [Publications] 横山隆: "術後感染症のempiric therapy"消化器外科. 23. 317-321 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 横山隆: "術後感染症の実態-最近の動向-"外科治療. 82. 513-519 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 横山隆: "急性腹症における抗菌薬の選択"消化器外科Nursing. 5. 830-836 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 横山隆: "EBMに基いた予防抗菌薬投与"Infection Control. 9. 774-778 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 竹末芳生: "術後感染防止の立場からみたドレナージ法"消化器外科. 23. 291-295 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 竹末芳生: "外科領域感染症に対する薬剤使用状況と耐性緑膿菌の発現頻度"日本化学療法学会雑誌. 48. 396-400 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 竹末芳生: "術後感染発症阻止薬の選択理論と実際 下部消化管手術"臨床外科. 55. 835-840 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakashita Y: "Generation of pro-inflammatory and anti-inflamamtory cytokines in the gut in zaymozan-induced peritonitis"Hiroshima J Med Sci. 49. 43-48 (2000)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takesue Y., et al.: "A brief course of colon preparation with oral antibiotics."Surg Today. 30. 112-116 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Sakashita Y., et al.: "Generation of pro-inflammatory and anti-inflamamtory cytokines in the gut in zaymozan-induced peritonitis"Hiroshima J Med Sci. 49. 43-48 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Ohge H., et al.: "An additional dose of Cefazolin for intraoperative prophylaxis"Surg Today. 29(12). 1233-1236 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Santo T., et al.: "Analysis of mec regulator genes in clinical methicillin-resistant Staphylococcus aurens isolates according to the production of coagulase, types of enterotoxin, and toxic shock syndrome toxin-1."Hiroshima J Med Sci. 48(2). 949-56 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takesne Y., et al.: "Changes in intestinal flora after administration of panipenem/betamipron or sulbactum/cefoperazone for treatment of postoperative infection in gastrectomy patients"J Infect Chemother. 5(1). 52-57 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yokoyama T., et al.: "Emerging og vancomycin resistant hospital stranis - mechanism of vancomycin resistance expression"Surgery Frontier. 6(1). 49-53 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yokoyama T., et al.: "Surgical infections"The Experiment & Therapy. 653. 20-24 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takesue Y., et al.: "Clinical use of antimicrobial agents in the infections by Pseudomonas aeruginosa"Nihongekakansensyoukenkyu. 11. 219-222 (1999)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Yokoyam T., et al.: "Empirie therapy for postoperative infection in the gastroenterological surgery"Gastroenterological surgery. 23. 317-321 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takesue Y., et al.: "The role of drainage in the prevention of postoperative infection"Gastroenterological surgery. 23. 291-295 (2000)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] Takesue Y., et al.: "Relationship of clinical use of antimicrobial agems and antibiotic susceptibility of Pseudomonas aeruginosa in a surgical department"Jpn.J.Chemother. 48. 396-400

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2000 Final Research Report Summary
  • [Publications] 横山隆: "術後感染症のempiric therapy"消化器外科. 23・3. 317-321 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 横山隆: "術後感染症の実態-最近の動向-"外科治療. 82・5. 513-519 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 横山隆: "急性腹症における抗菌薬の選択"消化器外科Nursing. 5・6. 830-836 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 横山隆: "EBMに基いた予防抗菌薬投与"Infection Control. 9・8. 774-778 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 竹末芳生: "術後感染防止の立場からみたドレナージ法"消化器外科. 23・3. 291-295 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 竹末芳生: "外科領域感染症に対する薬剤使用状況と耐性緑膿菌の発現頻度"日本化学療法学会雑誌. 48・6. 396-400 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 竹末芳生: "術後感染発症阻止薬の選択理論と実際 下部消化管手術"臨床外科. 55・7. 835-840 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Sakashita Y: "Generation of pro-inflammatory and anti-inflammatory cytokines in the gut in zaymozan-induced peritonitis"Hiroshima Journal of Medical Science. 49・1. 43-48 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 横山 隆: "感染防止とゾーニング"臨床透析. 92巻. 876-885 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] 横山 隆: "バンコマイシン耐性院内感染菌の出現-耐性のメカニズム-"Surgery Frontier. 6巻. 49-53 (1999)

    • Related Report
      1999 Annual Research Report
  • [Publications] Takesue Y: "A Brief Course of Colon Preparation with Oral Antibiotics"Surg Today. 30(2). 112-116 (2000)

    • Related Report
      1999 Annual Research Report

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Published: 1999-04-01   Modified: 2016-04-21  

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