| Budget Amount *help |
¥3,600,000 (Direct Cost: ¥3,600,000)
Fiscal Year 2000: ¥700,000 (Direct Cost: ¥700,000)
Fiscal Year 1999: ¥2,900,000 (Direct Cost: ¥2,900,000)
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| Research Abstract |
To elucidate the clinical use of genotyping for nosocomical infections of Pseudomonas aeruginosa (P.aeruginosa), we examined genotyping and serotyping of imipenem-resistant Pseudomonas aeruginosa (IRPA) isolated in our surgical ward. In 428 P.aeruginosa strains clinically isolated from the patients between 1987 and 1998, serotyping and genotyping were performed by the slideagglutination test and pulsed-field gel electrophoresis (PFGE), respectively. Suseeptibility ageinst imipenem was determined according to the NCCLS guidelines. Overall, the incidence of IRPA infection was 21.5%. The annual incidences of IRPA infections were particularly high in 1990, 1991 and 1994 (34-47%). Serologically, group-F strains were prevalent between 1990 and 1991, and group-B strains in 1994. In PFGE analysis, the identical pattern was shown in group-F strains, and three patterns were detected for group-B strains, suggesting that these outbreaks raged as nosocomial infections. By appropriate use of carbapenem antibiotics after 1995, the incidence of IRPA infections was reduced (14.6%) and neither serotyping nor PFGE analysis detected distinct outbreaks. However, computed dendrogram derived from digitized PFGE patterns revealed some sporadic nosocomial infections limited to "patient-to-patient". Although serotyping can be advocated for detecting outbreaks of nosocomial infections. PFGE may be useful for survey of "patient-to-patient" limited infection before outbreaks of nosocomial infections.
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