• Search Research Projects
  • Search Researchers
  • How to Use
  1. Back to previous page

Study of the pancreas specific glycopeptides for diagnosis of the pancreas cancer

Research Project

Project/Area Number 11671201
Research Category

Grant-in-Aid for Scientific Research (C)

Allocation TypeSingle-year Grants
Section一般
Research Field Digestive surgery
Research InstitutionHirosaki University

Principal Investigator

YOSHIHARA Syuichi  Hirosaki University, School of Medicine, Assistant Professor, 医学部, 講師 (00261454)

Co-Investigator(Kenkyū-buntansha) TOYOKI Yoshikazu  Hirosaki University, School of Medicine, Instructor, 医学部・附属病院, 助手 (70301025)
SASAKI Mutsuo  Hirosaki University, School of Medicine, Professor, 医学部, 教授 (10005077)
柴田 滋  弘前大学, 医学部・附属病院, 医員
Project Period (FY) 1999 – 2000
Project Status Completed (Fiscal Year 2001)
Budget Amount *help
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,400,000 (Direct Cost: ¥1,400,000)
Fiscal Year 1999: ¥2,100,000 (Direct Cost: ¥2,100,000)
Keywordshuman pancreatic juice / panceas cancer / tumor maker / glycopeptides / フコース / 糖ペプチド
Research Abstract

Introduction
Prognosis of the pancreatic cancer is extremely poor. Poor prognosis of this disease partially consists in difficulty of early diagnosis for it. More sensitive tumor marker is necessary for early diagnosis of it.
Recently we have isolated fucose-rich glycopeptide from human pancreatic juice. AH glycopeptides are found to be free of sialic acid and galactosamine but contained unusually high content of fucose. Structures in these glycopeptides are very rare in the human body. In this study, we intend to make the relationship in these glycopeptides and pancreatic cancer clear.
Purification and immunization of these glycopeptides
Three glycopeptides (GP-2, GP-3 and GP4) were purified employing ion exchange chromatography and gel filtration. GP-2 had a tetraantennary structure with five L-fucose residues. We tried to make polyclonal antibody for this glycopeptides (GP-2). Antigen (Peptide-carrier conjugation) was dissolved in phosphate-buffered saline and emulsified in complete Freund's adjuvant. For antigen, 2 rabbits were immunized intradermally with 1 mg of antigen, with booster immunizations given intramuscularly every 2 weeks in an identical manner except that incomplete Freund's adjuvant was used. Anti-glycopeptide antibody responses in the serum were evaluated using an ELISA procedure.
Immunochemical Staining
Frozen sections (4-6 μm) of the pancreas were blocked with normal goat serum , and subsequently incubated with primary antibodies diluted. Secondary reagents were biotinylated goat-anti-rabbit Ig. Sections were counterstained with hematoxyline. Organelle of the acinar cell were partially stained.
Concentration of the serum
Serum concentrations were measured in normal control group and pancreatic cancer group by ELISA. Differentiations of serum concentration (173.4 vs. 146.4 μg/dl) in control group and cancer group were revealed.

Report

(3 results)
  • 2001 Final Research Report Summary
  • 2000 Annual Research Report
  • 1999 Annual Research Report
  • Research Products

    (12 results)

All Other

All Publications (12 results)

  • [Publications] Ishido, K., et al.: "Enzymatic attachment of glycosaminoglycan to peptide using the sugar chain transfer reaction with end-β-xylosidase"J Biol Cem. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshihara, S., et al.: "Fine structural analysis of Fucose-rich glycopeptides from human pancreatic juice using 500-MHz ^1H-NMR spectroscopy"Hirosaki Med J. (in press). (2002)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshihara, S., et al.: "New Development in Glycomedicine. In : Endo M, Harata S, Saito Y, Munakata A, Sasaki M, Tsuchida S, editors"Elsevier Science B.V. (Amsterdam). 7 (2001)

    • Description
      「研究成果報告書概要(和文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Ishido, K., et al.: "Enzymic attachment of glycosaminoglycan to peptide using the sugar chain transfer reaction with end-β-xysidase"J. Biol. Chem.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshihara, S., et al.: "Fine structure analysis of Fucose-rich glycopeptide from human pancreatic juice using 500-MHz H-NMR spectroscopy"Hirosaki Med. J.. (in press). (2002)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Yoshihara, S., et al.: "Chemical structure of the carbohydrate moiety of Fucose-rich glycopeptides from human pancreatic Juice, New Development in glycomedicine. In:Endo et al, editors"Elsevier Science (Amsterdam). (2001)

    • Description
      「研究成果報告書概要(欧文)」より
    • Related Report
      2001 Final Research Report Summary
  • [Publications] Syuichi Yoshihara: "Structure analysis of fucose-rich glycopeptides from human pancreatic juice"Hirosaki Medical Journal. (掲載予定).

    • Related Report
      2000 Annual Research Report
  • [Publications] Syuichi Yoshihara: "Laparoscopic treatment for biliary ascariasis"Sergical Laparoscopy Endoscopy & Percutaneous Techniques. 10. 103-105 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 佐々木睦男: "生体肝移植術における拡大肝左葉および拡大左外測区域切除術のコツ"消化器外科. 23. 1479-1484 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] 佐々木睦男: "経十二指腸乳頭括約筋形成術"胆と膵. 21. 797-799 (2000)

    • Related Report
      2000 Annual Research Report
  • [Publications] Syuichi Yosihara: "Changes in Glycosaminoglycan of rat liver"Journal of Hepatology. (inpress).

    • Related Report
      1999 Annual Research Report
  • [Publications] Syuichi Yosihara: "Structure analysis of Fucose-rich glycopeptides from Human pancreatic juice"Hirosaki Medical Journal. (inpress).

    • Related Report
      1999 Annual Research Report

URL: 

Published: 1999-04-01   Modified: 2016-04-21  

Information User Guide FAQ News Terms of Use Attribution of KAKENHI

Powered by NII kakenhi