| Project/Area Number |
11671203
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Tohoku University |
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Principal Investigator |
NAITO Hiroo Tohoku Univ.Hospital Lecturer, 医学部・附属病院, 講師 (90180223)
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| Co-Investigator(Kenkyū-buntansha) |
FUKUSHIMA Kohei Tohoku Univ.Graduate School of Medicine Research Associate, 大学院・医学系研究科, 助手 (20271900)
FUNAYAMA Yuji Tohoku Univ.Hospital Lecture Research Associate, 医学部・附属病院, 講師 (50192315)
SASAKI Iwao Tohoku Univ.Graduate School of medicine Professor, 大学院・医学系研究科, 教授 (60125557)
SASAKI Kazuyuki Nisshin Pharm Co. Chief researcher, 主任研究員
YANAIHARA Noboru Yanaihara institute Chief researcher, 主任研究員
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| Project Period (FY) |
1999 – 2000
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| Project Status |
Completed (Fiscal Year 2000)
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| Budget Amount *help |
¥3,500,000 (Direct Cost: ¥3,500,000)
Fiscal Year 2000: ¥1,100,000 (Direct Cost: ¥1,100,000)
Fiscal Year 1999: ¥2,400,000 (Direct Cost: ¥2,400,000)
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| Keywords | GLP-2 / intestinal trophic effect / ODC activity / IEC-6 |
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| Research Abstract |
Plasma concentralion of GLP-2, histological changes of intestinal mucosa were investigated in rats with ileo-Jejunal Transposition(IJT)that show hyperenteroglucagonemia. Then, the efferct of external administration of glicenlin, GLP-1, and GLP-2 were evaluated regarding to the trophic effect on intestinal mucosa both in vitro and In vivo. The results were obtained as fonows 1)Plasma levels of GLP-2 in IJT rats were significantly higher than control rats showing the marked increase of villous heights of jeuno-ileal mucosa. 2)Extrnal administralion of GLP-2 significantly enhances the ODC activity in both jejuno-ielal mucosa in normal rats, which indicates the early phase trophic effect. The plasma
Levels of GLP-2 were almost same as the levels of those in rats with IJT.3)Both the ODC activities and tritium thymidine incorporation of IEC-6 were significantly stimulated by administration of GLP-2, which produces higher concentration of GLP-2 in the medium than the plasma concentrations of IJT rats. 4)Glicentin has the similar effect as shown in the experiments performed by the using GLP-2, however those effects of glicentin were less significant than GLP-2. 5)GLP-1 has no stimulating effects on parameters as described above both in vitro and vivo.
These results indicate a possible application of GLP-2 to the treatment of intestinal ulcer healing in patients with Crohn's disease. Moreover, it may be a new strategy to use of IJT in the surgical treatment of Crohn's disease.
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