| Project/Area Number |
11671210
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Single-year Grants |
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| Section | 一般 |
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| Research Field |
Digestive surgery
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| Research Institution | Gunma University |
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Principal Investigator |
ASAO Takayuki Gunma University, Faculty of Medicine, Assistant Professor, 医学部, 助教授 (40212469)
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| Co-Investigator(Kenkyū-buntansha) |
TAKENOSHITA Sei-ichi Fukushima Medical University, Faculty of Medicine, Professor, 医学部, 教授 (10167489)
KUWANO Hiroyuki Gunma University, Faculty of Medicine, Professor, 医学部, 教授 (90186560)
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| Project Period (FY) |
1999 – 2002
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| Project Status |
Completed (Fiscal Year 2002)
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| Budget Amount *help |
¥3,100,000 (Direct Cost: ¥3,100,000)
Fiscal Year 2002: ¥500,000 (Direct Cost: ¥500,000)
Fiscal Year 2001: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 2000: ¥800,000 (Direct Cost: ¥800,000)
Fiscal Year 1999: ¥1,000,000 (Direct Cost: ¥1,000,000)
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| Keywords | Glycosyltransferase / Glycochain / fucosyltransferase / mutation / metastasis / fucosyl transferase / 糖転移酵素 / フコシル糖鎖 / 癌の転移 / 遺伝子変化 / 遺伝子異変 |
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| Research Abstract |
1. We have previously reported that the elevated activities of serum alpha 1,3fucosyltransferase reverted to normal levels after curative removal of the tumors. To determine the origin of elevated serum alpha 1,3fucosyltransferase, blood samples were obtained from both the drainage vein and the artery in patients with different stages of colorectal cancer at surgery. The enzyme levels in all samples from the drainage vein were found to be higher than the levels in the artery that fed the tumor. Hence, the origin of elevated alpha1,3fucosyltransferase in serum was thought to be the tumor rather than the liver that is the normal source of serum alpha1,3fucosyltransferase. When serum samples not only from colorectal cancer patients but also from patients with gastric, liver, lung, pancreas, bladder and esophagus cancer were treated with anti-FUTVI antibody, the measured activities of alphal,3fucosyltransferase were markedly reduced. Further, secretion of alpha1,3fucosyltransferase from human colorectal carcinoma cells was also detected in the culture medium by Western immuno-blot analysis with anti-FUTVI antibody.
2.Cancer-related changes of glycoforms in α1-acid glycoprotein
The Netherlands. Abstractal-Acid glycoprotein (AGP), is a major serum glycoprotein with highN-linked sugar chains. Glycoforms of AGP were analyzed in sera from cancethe aid of crossed affinoimmunoelectrophoresis using ConA, AAL and anti-Achanges of their glycoforms were observed during the postoperative periodtheir branching and the extent of fucosylation in patients with advancedaccord with their clinical conditions.
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